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Phospho-FGFR4 (Tyr754) Rabbit Polyclonal Antibody#abs139979

Please note that the mentioned price is only provided for your reference. For detailed pricing information, kindly get in touch with our seller Vecent. Western blot analysis FGFR4 (Phospho-Tyr754) using PDGF treated NIH-3T3 whole cell lysates This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs139979-100ug	1-2 Weeks	301.0
abs139979-50ug	1-2 Weeks	201.0

Please note that the mentioned price is only provided for your reference. For detailed pricing information, kindly get in touch with our seller Vecent.

Overview
catalog	abs139979
Other names	The CD334 antigen, also known as FGFR-4, Fgfr4, or tyrosine kinase related to fibroblast growth factor receptor, is a protein tyrosine kinase that plays a critical role in cell growth and development. It is a hydroxyaryl protein kinase that is related to other members of the fibroblast growth factor receptor family, such as FGFR-1, FGFR-2, and FGFR-3. CD334 is expressed in a variety of tissues throughout the body, including the liver, pancreas, breast, and prostate. It is involved in a number of cellular processes, such as differentiation, proliferation, and migration, and is implicated in several human diseases, including cancer and bone disorders. The JTK 2 or JTK2 gene encodes the FGFR4 protein. Its clinical significance is still being studied, although it has been found to be involved in various types of cancer and is being explored as a potential target for cancer therapy. In summary, CD334 or FGFR-4 is a key player in cell growth and development that is expressed in various tissues and implicated in human disease. Its role and clinical relevance continue to be studied, offering the possibility of improved diagnosis and treatment for various conditions.
Source	Rabbit
Specificity	The Phospho-Tyr754 Antibody specifically recognizes FGFR4 in its phosphorylated form at Tyr754 and can accurately detect the endogenous levels of this protein. It is crucial to emphasize that this antibody only binds to FGFR4 when it is phosphorylated at Tyr754. By utilizing this antibody, researchers are able to determine the presence and amount of phosphorylated FGFR4 in various biological samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Sheep;Dog;Bovine;Horse
Antigen	FGFR4
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A newly developed peptide has been synthesized from human FGFR4 that targets the phospho-tyrosine residue at position 754. The peptide is highly similar to the original sequence and is designed to modulate the activity of the FGFR4 signaling pathway. This novel approach has the potential to provide new insights into the regulation of cellular processes and to pave the way for the development of new therapeutic strategies.
MW	87.9kDa
Properties
Concentration	1mg/ml
purification	The purified rabbit serum was utilized to obtain the antibody through a series of chromatography processes on both phospho- and non-phospho-peptide affinity columns by employing the affinity purification technique. The resulting content is expected to be highly similar to the original statement while ensuring that the text has unique phrasing and structure.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, the product should be kept at a temperature of -20 °C for a period of one year. It is important to avoid repeated cycles of freezing and thawing in order to maintain product integrity. Taking these precautions will help to ensure the highest quality and effectiveness of the product over time.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
Tissue specificity	Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
Posttranslational modification	N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19.Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated.Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.
Celluar localization	Endoplasmic reticulum;Endosome;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P22455