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Phospho-GCN2 (Thr899) Rabbit Polyclonal Antibody#abs139983

Description

Catalog-specification	Delivery time	USD price
abs139983-100ug	1-2 Weeks	301.0
abs139983-50ug	1-2 Weeks	201.0

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Overview
catalog	abs139983
Other names	Eukaryotic Translation Initiation Factor 2 alpha kinase 4, also known as Eif2ak4, is an essential protein involved in the regulation of translation initiation in eukaryotes. It is a member of the GCN2 family of kinases and plays a crucial role in the cellular response to stress and nutrient deprivation. The GCN2 protein, encoded by the Eif2ak4 gene, functions as a master regulator of protein synthesis. It phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α), leading to the repression of global protein synthesis and the initiation of stress-responsive gene expression. This mechanism allows cells to adapt to adverse conditions by conserving energy and redirecting resources towards stress-related processes. KIAA1338, a gene synonym for Eif2ak4, is expressed in a variety of tissues and is highly conserved across different species. Its expression is regulated by various cellular signals and can be induced by stressors such as amino acid starvation, viral infection, and endoplasmic reticulum stress. MGCN2, another name for Eif2ak4, is a key player in the integrated stress response pathway. This pathway ensures cellular survival under stress conditions by coordinating various cellular processes, including autophagy, amino acid metabolism, and immune response. Understanding the function and regulation of Eif2ak4 and its protein product, GCN2, is crucial for deciphering the intricate mechanisms that govern cellular response to stress and nutrient availability. Further research on this protein and its associated pathways may provide valuable insights into the development of therapeutic strategies for various diseases, including cancer, neurodegenerative disorders, and metabolic disorders.
Source	Rabbit
Specificity	The GCN2 (Phospho-Thr899) Antibody is specifically designed to detect levels of GCN2 that have been phosphorylated at Thr899. This antibody is highly specific and does not detect non-phosphorylated GCN2. It is able to detect endogenous levels of GCN2, making it a powerful tool for researchers studying various cellular processes. By using this antibody, researchers can better understand the role of GCN2 in various signaling pathways and its potential as a therapeutic target.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Sheep;Dog;Horse;Bovine
Antigen	GCN2
Application	For Western blotting, a recommended dilution is 1:1000-3000, while for immunohistochemistry, a dilution of 1:50-1:200 is recommended. When it comes to ELISA using peptides, a dilution range of 1:20000-1:40000 is ideal. These dilutions have been chosen based on their efficacy and reliability in producing accurate and consistent results. It is important to note that different applications may require different dilutions, so it is always best to consult the manufacturer's instructions or seek advice from an expert in the field before proceeding with any experimental protocols.
Immunogen	A peptide has been synthesized from human GCN2, specifically from the phosphorylated threonine residue at position 899. The peptide is designed to be highly similar to the original protein sequence.
MW	187kDa
Properties
Concentration	1mg/ml
purification	The process of obtaining the antibody involved purifying rabbit serum and conducting affinity purification using phospho- and non-phospho-peptide affinity columns in sequential chromatography. To generate highly similar content based on the original text information, the following can be rearranged: Sequential chromatography was employed to purify the antibody, which was derived from purified rabbit serum. Affinity purification was carried out using both phospho- and non-phospho-peptide affinity columns.
Clonality	Polyclonal Antibody
Stability & Storage	It is important to store the product at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to multiple freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:25329545). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).
Tissue specificity	Widely expressed (PubMed:10504407). Expressed in lung, smooth muscle cells and macrophages (PubMed:24292273).
Posttranslational modification	Autophosphorylated; autophosphorylation on Thr-899 is increased upon amino acid starvation and in UV irradiation cells and inhibited in presence of IMPACT.
Celluar localization	Cytosol;
UniPort	Q9P2K8