Phospho-EPHB1/2/3/4 (Tyr600/602/614/596) Rabbit Polyclonal Antibody#abs139974
Please note that the price provided is only for your reference. For more detailed pricing information, we kindly ask that you reach out to our seller Vecent. Thank you for your understanding. Western blot analysis EPHB1/2/3/4 (Phospho-Tyr600/602/614/596) using UV treated 293 whole cell lysates...
Description
| Catalog-specification | Delivery time | USD price |
abs139974-100ug | 1-2 Weeks | 301.0 |
abs139974-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is only for your reference. For more detailed pricing information, we kindly ask that you reach out to our seller Vecent. Thank you for your understanding.
| Overview | |
catalog | abs139974 |
| Other names | There are various proteins known as EPH receptors that are involved in key biological functions such as development, tissue patterning, and synaptic plasticity. Among the EPH receptors are EPH receptor B1, EPH receptor B2, and EPH receptor B3. Additionally, there are other EPH-like kinases such as EK2, EK5, and EK6. These kinases all belong to the EPH family and share a similar structure consisting of a tyrosine kinase domain and an extracellular domain that binds to ephrin ligands. EPH receptor B1, also known as EPHB1 or HEK6, is expressed in various tissues including brain, kidney, heart, and lung. It is involved in neural development and has been associated with certain types of cancer. Similarly, EPH receptor B2, or EPHB2, is also involved in development and has been implicated in cancer metastasis. EPH receptor B3, or EPHB3, plays a role in axon guidance and is important for the development of the nervous system. EK2, also known as embryonic kinase 2 or EPH-like tyrosine kinase 2, is involved in tissue patterning during development. EK5, or EPH-like kinase 5, is expressed in the nervous system and has been associated with neuronal differentiation. EK6, or EPH-like kinase 6, is also expressed in the nervous system and has been shown to play a role in synaptic plasticity. Overall, these EPH receptors and kinases are important regulators of various biological processes and are potential targets for therapeutic interventions in cancer, neurological disorders, and developmental abnormalities. |
| Source | Rabbit |
| Specificity | The EPHB1/2/3/4 (Phospho-Tyr600/602/614/596) Antibody specifically recognizes the phosphorylated form of endogenous EPHB1/2/3/4 proteins at Tyr600/602/614/596. This antibody is designed to detect only the phosphorylated state of these proteins. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Zebrafish;Chicken;Rabbit;Pig;Dog;Horse;Bovine;Sheep; |
| Antigen | EPHB1/2/3/4 |
| Application | WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | A synthesized peptide derived from human EPHB1/2/3/4 (Phospho-Tyr600/602/614/596). |
| MW | 110KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity). |
| Tissue specificity | Preferentially expressed in brain. |
| Posttranslational modification | Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2 domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL.Ubiquitinated; (EFNB1)ligand-induced poly- and/or multi-ubiquitination by CBL is regulated by SRC and leads to lysosomal degradation. |
| Celluar localization | Cytosol;Endoplasmic reticulum;Endosome;Extracellular region or secreted;Plasma Membrane; |
| UniPort | P54762/P29323/P54753/P54760 |
Western blot analysis EPHB1/2/3/4 (Phospho-Tyr600/602/614/596) using UV treated 293 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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