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Rabbit Anti-EPHA3 Polyclonal Antibody#abs131350

Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of EPHA3 expression in 293 cells. This product is for research use only, not for use in diagnostic prodecures or...

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Description

Catalog-specification	Delivery time	USD price
abs131350-50ug	1-2 Weeks	201.0
abs131350-100ug	1-2 Weeks	301.0

Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs131350
Description	This receptor specifically binds to ephrin-A2, -A3, -A4, and -A5, which are members of the ephrin-A family. It has the potential to contribute to lymphoid function. Let's explore the significance of this receptor in mediating interactions between ephrin-A proteins and its potential role in lymphoid activity.
Other names	Eph receptor A3 is a protein encoded by the TYRO4 gene. It is also known as EK4, EPH-like kinase 4, Mek4, and human embryo kinase 1. The receptor tyrosine kinase HEK is involved in cell signaling pathways that regulate cell growth, differentiation, and migration. Additionally, it plays a crucial role in neural development and maturation. The protein belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. It binds to members of the ephrin-A family, which are involved in many developmental processes, and the interaction between the receptor and its ligand leads to the phosphorylation of the receptor and downstream signaling events. Mutations in the TYRO4 gene have been associated with several cancers, including lung cancer, ovarian cancer, and gastric cancer. In addition, it has been implicated in a variety of other diseases, including Alzheimer's disease, epilepsy, and cardiovascular disease. In summary, Eph receptor A3 is a crucial signaling molecule that plays a vital role in several physiological and pathological processes. Further research into its function in various disease states may lead to the development of novel therapeutic strategies.
Source	Rabbit
Specificity	The EPHA3 Antibody is capable of detecting the overall levels of endogenous EPHA3 protein. This indicates that the antibody can recognize and bind to the target protein present in its natural form.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Rabbit;Pig;Dog;Horse;Bovine;Sheep;
Antigen	EPHA3
Application	The dilution ratios for WB, IHC, and ELISA(peptide) are as follows: - WB: 1:500-1:2000 - IHC: 1:50-1:200 - ELISA(peptide): 1:20000-1:40000 To obtain accurate results, it is crucial to dilute the samples properly according to these ratios. WB should be diluted at a ratio of 1:500-1:2000, while IHC requires a dilution of 1:50-1:200. For ELISA(peptide), a dilution of 1:20000-1:40000 is recommended. Following these guidelines will ensure reliable data and optimal performance of these techniques.
Immunogen	A synthesized peptide derived from human EPHA3.
MW	110 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. A highly similar content can be generated by rephrasing the original text as follows: The purification process of the antiserum was carried out by means of peptide affinity chromatography, in which SulfoLink™ Coupling Resin was utilized for the purpose of purification.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Tissue specificity	Widely expressed. Highest level in placenta.
Posttranslational modification	Autophosphorylates upon activation by EFNA5. Phosphorylation on Tyr-602 mediates interaction with NCK1. Dephosphorylated by PTPN1.
Celluar localization	Endosome;Extracellular region or secreted;Plasma Membrane;
UniPort	P29320