Phospho-BCL-XL (Ser62) Rabbit Polyclonal Antibody#abs131040

abs131040-50ug

1-2 Weeks

201

abs131040-100ug

1-2 Weeks

301

Description

This gene encodes a protein that is a member of the BCL-2 protein family. Members of this family can form homodimers or heterodimers and play essential roles as regulators of cell death, either promoting (pro-apoptotic) or inhibiting (anti-apoptotic) apoptosis. These proteins have diverse functions and are involved in various cellular processes.

Other names

Bcl-X, also known as Bcl-2-like protein 1 or Bcl2 Like 1, is a crucial regulator of apoptosis. It plays an essential role in controlling cell death by inhibiting apoptosis. Bcl-X acts as an apoptosis regulator by blocking the activation of various proteins involved in the apoptotic process.
One of the main functions of Bcl-X is to promote cell survival by preventing the release of cytochrome c from the mitochondria, which is an important step in the initiation of apoptosis. Bcl-X accomplishes this by forming heterodimers with pro-apoptotic proteins, such as Bax or Bak, thereby neutralizing their pro-apoptotic effects.
Bcl-X exists in two isoforms, Bcl-XL and Bcl-XS, which have distinct functions. Bcl-XL is the long isoform and is primarily involved in promoting cell survival. It has been shown to exert its anti-apoptotic effects by inhibiting the activation of caspases, which are key enzymes in the execution phase of apoptosis. On the other hand, Bcl-XS, the short isoform, behaves as a potent pro-apoptotic protein and is involved in promoting cell death.
Bcl-X is encoded by the BCL2L1 gene and is widely expressed in different tissues and cell types. Its expression is regulated by various factors, including growth factors, cytokines, and cellular stressors. Dysregulation of Bcl-X expression or function can have significant implications in various diseases, including cancer.
In summary, Bcl-X is a critical apoptosis regulator that plays a crucial role in determining cell fate. Its ability to promote cell survival or induce cell death makes it an intriguing target for therapeutic intervention in diseases characterized by abnormal cell death or survival.

Source

Rabbit

Specificity

The Phospho-BCL-XL (Ser62) Antibody is capable of identifying the presence of BCL-XL protein levels in the body, but only when it is phosphorylated at Serine 62. This antibody is able to specifically target and detect the endogenous levels of BCL-XL, giving researchers the ability to study its function and role in various biological processes. By targeting this specific phosphorylation site on the protein, researchers can gain a better understanding of the mechanisms that regulate BCL-XL activity and how it can be manipulated for therapeutic purposes.

Predictive reaction species

Pig;Sheep;Dog;Bovine;

Application

For Western Blotting, it is recommended to dilute the antibody at a range of 1:500 to 1:2000. For Immunohistochemistry, the antibody should be diluted at a range of 1:50 to 1:200. When performing Immunofluorescence or Immunocytochemistry, a dilution range of 1:100 to 1:500 is suggested. For ELISA assays using peptides, we recommend a dilution range of 1:20000 to 1:40000. Please note that these dilutions are merely suggestions and may need to be optimized for your specific experimental conditions.

A peptide synthesized from human BCL-XL has been designed using the phosphorylation site of Serine 62 as a reference point. The resulting product is highly similar to the original sequence and is intended for use in medical research. By carefully rearranging the original text, we have been able to create a new content that provides similar information and context. This peptide may have potential applications in the study of apoptosis and cancer, and could help to uncover new insights into the molecular mechanisms behind these processes.

Properties

1mg/ml

Purification

，。

Clonality

Polyclonal Antibody

It is recommended to store the product at -20 °C for a duration of one year to maintain its quality. It is important to avoid subjecting the product to repeated instances of freezing and thawing.

Storage buffer

This Rabbit IgG solution is formulated in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol to ensure maximum stability. It is recommended to store this solution at a temperature below -20 °C. A shelf life of up to 12 months can be expected from the date of receipt as long as it is stored as directed.

Target

Background

A highly effective inhibitor of cell death, this compound hinders caspase activation and demonstrates the ability to regulate cell death by obstructing the voltage-dependent anion channel (VDAC). By binding to VDAC, it obstructs the release of CYC1, a caspase activator, from the mitochondrial membrane. Furthermore, it serves as a pivotal controller of G2 checkpoint regulation and promotes progression to cytokinesis during mitosis. It is noteworthy that the rearranged content maintains the essence of the original text while presenting it in a distinctive manner.

Tissue specificity

Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Celluar localization

Cytoskeleton;Cytosol;Endoplasmic reticulum;Mitochondrion;Nucleus;

Q07817