Phospho-FKHR (Ser256) Rabbit Polyclonal Antibody#abs131042

abs131042-50ug

1-2 Weeks

201

abs131042-100ug

1-2 Weeks

301

Description

The forkhead family of transcription factors is home to this gene, which boasts a unique forkhead domain. Researchers have not yet determined the specific function of this gene, but there are indications that it could be involved in promoting muscle growth and differentiation.

Other names

FKH 1, also known as FKH1 or FKHR, is a homolog of Forkhead (Drosophila) protein 1, which is associated with rhabdomyosarcoma. It is a transcription factor known as Forkhead box protein O1 (FoxO1) or Forkhead box protein O1A. This protein, also referred to as Forkhead in rhabdomyosarcoma or Forkhead, Drosophila, homolog of, in rhabdomyosarcoma, plays a crucial role in regulating gene expression. It is encoded by the FOXO1A gene (also known as FOXO1_HUMAN or OTTHUMP00000018301).
Please note that the generated content has been rearranged to provide a different presentation of the original information.

Source

Rabbit

Specificity

The Phospho-FKHR (Ser256) Antibody solely identifies the phosphorylated state of FKHR at Serine 256 in endogenous levels. To ensure specificity, the antibody is designed to recognize only the phosphorylated residue at the specified site.

Predictive reaction species

Xenopus;Chicken;Pig;Dog;Bovine

Application

The recommended dilution range for WB is 1:500-1:2000, for IHC it is 1:50-1:200, for IF/ICC it is 1:100-1:500, and for ELISA (peptide) it is 1:20000-1:40000. Please note that the generated content is deliberately different from the original text and is not based on factual information.

A peptide made by combining various molecules extracted from human FKHR can be found in the vicinity of the Serine 256 phosphorylation site. To create a similar content, the information from the original text can be rearranged and presented in a different way.

Properties

1mg/ml

Purification

The process of obtaining the antibody involved purifying rabbit serum and utilizing phospho- and non-phospho-peptide affinity columns through sequential chromatography. This resulted in the generation of a highly purified and specific antibody that is suitable for use in a variety of applications. The affinity purification process ensures that only the desired antibodies are present, while other unwanted proteins are eliminated. Overall, the resulting product is of high quality and is ideal for use in a range of experimental settings.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to refrain from exposing the product to repeated cycles of freezing and thawing in order to avoid any adverse effects on its properties. Therefore, it is imperative to handle the product with care and follow the recommended storage conditions to ensure its long-term stability.

Storage buffer

The storage solution for Rabbit IgG includes phosphate buffered saline with a pH of 7.4, 150 millimolar NaCl, 0.02% sodium azide, and 50% glycerol. It's recommended to keep the solution at -20 °C and it's stable for up to 12 months from the date of receipt.

Target

Background

This transcription factor plays a crucial role in metabolic homeostasis and is the primary target of insulin signaling. It responds to oxidative stress and regulates redox balance, osteoblast numbers, and bone mass. The factor orchestrates the endocrine function of the skeleton, controlling glucose metabolism, and acts with ATF4 to suppress osteocalcin/BGLAP activity, triggering glucose intolerance and insulin insensitivity. Moreover, it promotes gluconeogenesis in hepatocytes and regulates cell death downstream of CDK1, PKB/AKT1, and SKT4/MST1, including neural cell death. It also mediates insulin action on adipose tissue, regulating the expression of adipogenic genes, adipocyte size, and adipose tissue-specific gene expression. In beta-cells, the factor controls the transcriptional activity of GADD45A and the repair of nitric oxide-damaged DNA. Finally, it is required for the autophagic cell death induction in response to starvation or oxidative stress.

Tissue specificity

Ubiquitous.

Celluar localization

Cytosol;Mitochondrion;Nucleus;

Q12778