Rabbit Anti-NF-kappaB P100/p52 Polyclonal Antibody#abs131879
Please note that the price provided is for your reference only. For detailed pricing information, please reach out to our seller Vecent. It is important to mention that the generated content will be distinct from the original text, as we will employ a language model to create a different...
Description
| Catalog-specification | Delivery time | USD price |
abs131879-100ug | 1-2 Weeks | 301.0 |
abs131879-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for your reference only. For detailed pricing information, please reach out to our seller Vecent. It is important to mention that the generated content will be distinct from the original text, as we will employ a language model to create a different approach to communication, rather than relying on the way ChapGPT generates content.
| Overview | |
catalog | abs131879 |
Description | NFkB-p100 is a vital transcription factor and a member of the nuclear factor-kappaB (NFkB) group. It serves as the precursor of the p52 subunit, which binds to the kappa-B consensus sequence located in the enhancer region of genes. These genes play a critical role in immune response and acute phase reactions. NFkB-p100 is an essential factor for regulating gene expression, and its activity is tightly controlled in various physiological processes. Understanding its function is crucial in developing therapeutic strategies for inflammatory and autoimmune diseases. |
| Other names | The DNA binding factor KBF2, also known as CVID10, H2TF1, LYT 10, or NF kB2, is a critical transcription factor involved in immune system regulation. Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) or NFKB p52/p100 subunit, as it is also called, plays a key role in the activation of genes related to inflammation, cell proliferation, and survival. Its expression is essential for proper lymphocyte translocation and function. The nuclear factor Kappa B subunit 2 is a potent oncogene and plays a vital role in cancer development and progression. Studies have shown that aberrant expression of NFKB2 is associated with a variety of cancers, including lymphoma, lung, breast, and colon cancer. The p100 protein, the precursor of the p52 subunit, is often overexpressed in tumor cells, highlighting its potential importance as a therapeutic target. In summary, the NFKB2 gene and its protein products play crucial roles in immune system function, inflammation, and oncogenesis. Understanding the mechanisms behind its regulation and activation could shed light on the development of novel therapeutics for immune disorders and cancer. |
| Source | Rabbit |
| Specificity | The antibody for NF-kappaB p100/p52 is designed to detect the total amount of this protein present in the body. It can specifically identify the endogenous levels of NF-kappaB p100/p52 and help with the characterization of its roles and functions. This antibody can be an essential tool for researchers who are interested in studying the biology of NF-kappaB p100/p52 and related pathways. Its accuracy and sensitivity make it a valuable asset for various applications in the field of molecular biology and immunology. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Chicken;Rabbit;Pig;Dog;Bovine;Sheep; |
| Antigen | NF-kappaB p100/p52 |
| Application | The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IP and IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. It is important to follow these dilutions to ensure accurate and reliable results. |
| Immunogen | Peptide synthesis of a human NF-kappaB p100/p52 derivative has been successfully achieved. The synthesized peptide is a faithful representation of the original sequence, obtained by rearranging specific amino acid residues in accordance with the information contained within the given NF-kappaB p100/p52 peptide. |
| MW | 100kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | We employed SulfoLink™ Coupling Resin for peptide affinity chromatography in order to purify the antiserum. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. |
| Posttranslational modification | While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain. |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | Q00653 |
Western blot analysis of NF-kappaB p100/p52 expression in ovarycancer whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
This product is for research use only, not for use in diagnostic prodecures or in human.
Hot Tags: rabbit anti-nf-kappab p100/p52 polyclonal antibody#abs131879, China rabbit anti-nf-kappab p100/p52 polyclonal antibody#abs131879 suppliers
Send Inquiry
You Might Also Like
