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Rabbit Anti-HSL Polyclonal Antibody#abs131896

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Description

Catalog-specification	Delivery time	USD price
abs131896-50ug	In Stock	201.0
abs131896-100ug	In Stock	301.0

Overview
catalog	abs131896
Description	Hormone sensitive lipase is an important enzyme of the 'GDXG' family that is involved in the breakdown of triglycerides. It serves as a critical step in the process of lipolysis, which is the breakdown of fat molecules stored in adipose tissue and other tissues. Hormone sensitive lipase primarily works in adipose tissue and the heart, where it helps to convert stored triglycerides into free fatty acids. This process is essential for providing energy to the body and maintaining a healthy weight. In steroidogenic tissues, hormone sensitive lipase also plays an essential role in the production of steroid hormones. Here, it converts cholesteryl esters to free cholesterol, which is a critical building block for the synthesis of hormones such as testosterone and estrogen. In summary, hormone sensitive lipase is a versatile enzyme that plays a vital role in lipid metabolism and hormone production in the body.
Other names	The hormone-sensitive lipase, also known as HSL or LIPE, is an important enzyme that plays a vital role in lipid metabolism. It is responsible for breaking down stored triglycerides into free fatty acids and glycerol, which can then be used by the body as a source of energy. There are different isoforms of HSL, including the testicular isoform, which is specific to the testes. The HSL enzyme is regulated by various hormones, including insulin, glucagon, and adrenaline. When these hormones are released in response to different signals, they activate HSL, enabling it to break down triglycerides and release free fatty acids into the bloodstream. Defects in the HSL enzyme can lead to various metabolic disorders, such as obesity, diabetes, and dyslipidemia. Therefore, measuring the levels and activity of HSL can be useful in diagnosing and monitoring these conditions. Overall, the hormone-sensitive lipase is a crucial enzyme that plays a vital role in lipid metabolism and energy homeostasis. Its different isoforms and regulation by various hormones make it a complex enzyme, and understanding its function can help in the management of various metabolic disorders.
Source	Rabbit
Specificity	The antibody for HSL is capable of detecting total HSL at the endogenous levels within the body. To create similar content, one could state that the HSL antibody has the ability to identify the complete amount of HSL that naturally occurs in the body.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;Horse
Antigen	HSL
Application	The recommended dilutions for using this antibody in various applications are as follows: Western blotting should be performed at a dilution range of 1:500 to 1:2000, while the recommended dilutions for immunohistochemistry are 1:50 to 1:200. Immunofluorescence and immunocytochemistry require a dilution range of 1:100 to 1:500. Finally, for ELISA detection using a peptide, the antibody should be diluted between 1:20000 and 1:40000. By following these recommended dilutions, optimal results can be achieved with this antibody in various applications.
Immunogen	A synthesized peptide derived from human HSL.
MW	85kDa
Properties
Concentration	1mg/ml
purification	By utilizing SulfoLink™ Coupling Resin, the antiserum was able to undergo purification via peptide affinity chromatography. This procedure successfully removed any impurities from the antiserum, resulting in a highly purified end product that is ready for further analysis and utilization.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Posttranslational modification	Phosphorylation by AMPK may block translocation to lipid droplets.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	Q05469