Rabbit Anti-N-Cadherin Polyclonal Antibody#abs131133

abs131133-50ug

1-2 Weeks

201

abs131133-100ug

1-2 Weeks

301

Description

Cadherins serve as crucial cell adhesion proteins that rely on calcium for their function. These proteins have a strong tendency to bind to identical cadherins on neighboring cells, allowing for the establishment of connections between different cell types. This ability of cadherins to preferentially interact with cells of the same kind plays a vital role in the process of sorting heterogeneous cell populations. Notably, CDH2, a specific type of cadherin, is believed to participate in neuronal recognition mechanisms. It is suggested that CDH2 may regulate the density of dendritic spines in hippocampal neurons, which in turn has implications for neuronal connectivity and communication. Through its intricate functions, cadherins contribute significantly to the coordination and organization of diverse cell types in various biological contexts.

Other names

N-cadherin, also known as CDH2 or neuronal cadherin, is a calcium-dependent adhesion protein involved in cell-cell adhesion. It plays a crucial role in neuronal development and function. N-cadherin is encoded by the CDH2 gene and is expressed primarily in neurons. It is classified as a type 1 cadherin and is part of the cadherin superfamily. CD325 is another name for N-cadherin and is often used as a marker for this protein. In addition, N-cadherin is also referred to as N cadherin 1, neural cadherin, or CDw325 antigen. It is an essential molecule for maintaining the integrity and connectivity of neuronal networks.

Source

Rabbit

Specificity

Our antibody is capable of identifying the total amount of endogenous N-Cadherin protein present in the system. We are able to detect and measure the levels of this protein accurately using our specialized antibody.

Application

When it comes to the recommended dilutions for our protein, we suggest using a WB dilution of 1:500-1:1000. For IHC, we recommend a dilution range of 1:50-200, while for IF, we advise using a 1:200 dilution. Finally, for ELISA with a peptide, we recommend a dilution range of 1:20,000-1:40,000. It's important to note that these dilutions are based on the original text information and should be adjusted for optimal results in your specific experiment.

V-K-P-A-I is a five amino acid sequence that can be found near position 800-804 in the human N-Cadherin protein. This peptide sequence holds significance in various protein structures and interactions. By rearranging the original amino acid sequence, we can uncover new insights into the protein's function and interactions. For example, I-A-K-P-V is a potential rearrangement that could have an effect on protein folding or interaction at the cell membrane. Understanding the significance of these small peptide sequences can aid in the development of new drugs and therapies.

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography.

Clonality

Polyclonal Antibody

To ensure optimal storage, it is recommended to keep the product at -20°C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. Taking these precautions will help maintain the integrity and quality of the product over time.

Storage buffer

The recommended storage conditions for the Rabbit IgG in phosphate buffered saline are at -20 °C. This product is formulated in a solution with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this solution is stable for a period of 12 months starting from the date of receipt.

Target

Background

Cadherins are proteins that facilitate the attachment of cells to each other in a calcium-dependent manner. This process is homophilic in nature, allowing for the sorting of diverse cell types. One of the key roles of cadherins is to regulate the quiescence of neural stem cells. They achieve this by anchoring the cells to ependymocytes in the adult subependymal zone. However, this anchorage process is affected by cleavage via MMP24, resulting in the modulation of neural stem cell quiescence. Additionally, CDH2 may be involved in facilitating neuronal recognition mechanisms. In hippocampal neurons, CDH2 may also have a role in controlling dendritic spine density.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane;

P19022