Phospho-PKD1/PKC μ (Tyr463) Rabbit Polyclonal Antibody#abs131068

abs131068-50ug

1-2 Weeks

201

abs131068-100ug

1-2 Weeks

301

Description

The PKC family plays a crucial role in a variety of signal transduction pathways that are triggered by extracellular receptors. To learn more about these pathways, refer to the PRKCA (MIM 176960) for additional information and background context.

Other names

Protein kinase C mu, also known as nPKC D1 or nPKCmu, is a serine/threonine protein kinase encoded by the PRKD1 gene. It is a type of protein kinase D and is often referred to as PKC mu, PKC MU, or PKCM. The protein plays a crucial role in various cellular processes, including cell proliferation, differentiation, and survival. It is involved in signaling pathways that regulate cell growth, migration, and apoptosis. PKD1 is an important mediator of intracellular signaling and is implicated in several diseases, including cancer and neurological disorders. This highly conserved protein kinase is known for its diverse functions and is a subject of extensive research to unravel its intricate molecular mechanisms.

Source

Rabbit

Specificity

The antibody, Phospho-PKD1/PKC μ (Tyr463), is specifically designed to recognize and bind to PKD1/PKC μ protein only when it is phosphorylated at Tyrosine 463. This antibody enables the detection of the endogenous levels of PKD1/PKC μ protein in biological samples. By targeting this specific phosphorylation site, it provides a valuable tool for studying the functional regulation and signaling pathways involving PKD1/PKC μ protein.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

The recommended dilutions for WB range from 1:500 to 1:2000, for IHC they range from 1:50 to 1:200, for IF/ICC they range from 1:100 to 1:500, and for ELISA (peptide) it is suggested to use a dilution range of 1:20000 to 1:40000. It is important to note that these dilutions are based on the original text information and should be followed accordingly.

PKC μ, also known as protein kinase D1 (PKD1), is a vital component of human cells. One particular region of interest lies around the phosphorylation site of Tyrosine 463. To further explore the potential functions and effects of this region, a synthesized peptide derived from human PKD1/PKC μ is being studied. By analyzing and rearranging the given content, a highly similar representation can be generated, ensuring that it remains grounded in the original text information.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

To maintain the quality, store the product at a temperature of -20 °C for a period of one year. Make sure to avoid subjecting it to multiple cycles of freezing and thawing.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline at a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20 °C. The stability of this product is guaranteed for 12 months from the date of receipt. Please note that the content generated by the language model should be significantly different from the original text and should not follow the format of a conversation generated by ChapGPT.

Target

Background

PRKD1, a serine/threonine-protein kinase, is involved in various physiological processes such as cell survival, cell migration, cell differentiation, cell proliferation, and regulation of gene expression. It converts transient DAG signals into prolonged physiological effects downstream of PKC. PRKD1 phosphorylates several targets such as the epidermal growth factor receptor (EGFR), RIN1, HDAC7, cardiac troponin I (TNNI3), and transcription factor TFEB. The phosphorylation of these targets leads to various cellular responses, including the suppression of MAPK8/JNK1 activation, inhibition of RAF1 binding to GTP-bound form of Ras proteins, maintenance of the structural integrity of the Golgi membranes, activation of NF-kappa-B, and regulation of gene expression. PRKD1 is also involved in the regulation of VEGFA-induced angiogenesis, genotoxic-induced apoptosis, flagellin-stimulated inflammatory response, and pain transmission. In addition, PRKD1 plays a crucial role in the activated KRAS-mediated stabilization of ZNF304 in colorectal cancer cells. PRKD1 is a critical component of the signaling pathways that regulate various biological functions, and its dysregulation leads to the development of several diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders.

Tissue specificity

Up-regulated by the intestine-specific transcription factor CDX1 in a activated KRAS-dependent manner in colorectal cancer (CRC) cells (PubMed:24623306).

Celluar localization

Cytosol;Golgi apparatus;Plasma Membrane;

Q15139