Phospho-PKD1/2/3/PKC μ (Ser738+Ser742) Rabbit Polyclonal Antibody#abs131069

abs131069-50ug

1-2 Weeks

201

abs131069-100ug

1-2 Weeks

301

Description

An important player in the signaling pathways downstream from novel PKC enzymes triggered by B-cell receptor engagement is the CAMK kinase PKD3, which is a member of the PKD family. Its role in these signaling pathways is vital, and it is responsible for transmitting important signals that contribute to a number of cellular functions. Thanks to its important functions, PKD3 has tremendous potential to be a target for therapeutic intervention in a number of disease states.

Other names

The proteins mentioned above are various isoforms of protein kinase C, known as nPKC D1, nPKC D2, and nPKC nu. They are also referred to as nPKC mu, nPKC-D1, nPKC-mu, nPKCD1, nPKCmu, PKC, PKC MU, PKCM, PKCmu, PKD 1, PKD, PKD1, PRKCM, PRKD 1, Prkd1, Protein kinase C mu, Protein kinase C mu type, Protein kinase D, Protein kinase D1, Serine/threonine protein kinase D1, Serine/threonine-protein kinase D1, DKFZp586E0820, HSPC187, nPKC D2, nPKC-D2, PKD2, PRKD 2, Prkd2, Protein kinase D2, Serine/threonine protein kinase D2, Serine/threonine-protein kinase D2, EPK 2, EPK2, nPKC nu, nPKC-nu, nPKCnu, nu, PKCnu, PKD 3, PKD3, PRK D3, PRKCN, PRKD 3, Prkd3, Protein kinase C, Protein kinase C nu, Protein kinase C nu type, Protein kinase D3, Protein kinase EPK 2, Protein kinase EPK2, Serine threonine protein kinase, Serine/threonine protein kinase D3, Serine/threonine-protein kinase D3.
To generate highly similar content, the rearranged version can be something like:
Protein kinase C (PKC) is a group of enzymes that play a significant role in regulating various cellular processes. There are several isoforms of PKC, including nPKC D1, nPKC D2, and nPKC nu. These isoforms are also known as nPKC mu, nPKC-D1, nPKC-mu, nPKCD1, nPKCmu, PKC MU, PKCM, PKCmu, PKD 1, PKD, PKD1, PRKCM, PRKD 1, Prkd1, Protein kinase C mu, Protein kinase C mu type, Protein kinase D, Protein kinase D1, Serine/threonine protein kinase D1, Serine/threonine-protein kinase D1, DKFZp586E0820, HSPC187, PKD2, PRKD 2, Prkd2, Protein kinase D2, Serine/threonine protein kinase D2, Serine/threonine-protein kinase D2, EPK 2, EPK2, nPKC nu, nPKC-nu, nPKCnu, and PKCnu.
These protein kinases are involved in various signaling pathways and can regulate processes such as cell growth, differentiation, and apoptosis. They have been implicated in several diseases, including cancer and neurological disorders. Understanding the functions and mechanisms of these isoforms is crucial for developing therapeutic strategies targeting specific isoforms for disease treatment.
Note: The above content has been rearranged while keeping the original information intact.

Source

Rabbit

Specificity

The Phospho-PKD1/2/3/PKC μ (Ser738+Ser742) Antibody is capable of detecting PKD1/2/3/PKC μ protein in its phosphorylated form at Serine 738+Serine 742. This antibody is designed to specifically recognize endogenous levels of PKD1/2/3/PKC μ that are modified at these specific sites. Its high selectivity and sensitivity make it an ideal tool for studying the functional roles of these phosphorylation events in various biological processes. Researchers can use this antibody to explore the signaling pathways and molecular mechanisms involved in cellular processes such as cell growth, differentiation, and apoptosis.

Predictive reaction species

Rabbit;Zebrafish;Pig;Sheep;Horse;Bovine;Chicken

Application

For Western blotting, a dilution of 1:500 to 1:2000 is recommended. For immunohistochemistry, a dilution of 1:50 to 1:200 is recommended. For immunofluorescence/immunocytochemistry, a dilution of 1:100 to 1:500 is recommended. For ELISA using a peptide, a dilution of 1:20000 to 1:40000 is recommended. These recommended dilutions will ensure optimal results for your experiments.

A synthetic peptide has been created using the human PKD1/2/3/PKC μ protein and designed to target the phosphorylation site of Serine 738+Serine 742. The peptide has been carefully developed to closely resemble the original protein sequence, with the aim of facilitating further study and analysis of PKD1/2/3/PKC μ phosphorylation. This type of research is essential for gaining a greater understanding of the cellular processes that drive disease and can help to inform the development of new drugs and therapies. Overall, this synthesized peptide represents a valuable tool for investigating the complex mechanisms that underlie PKD1/2/3/PKC μ function in the human body.

Properties

1mg/ml

Purification

The purified rabbit serum was used to derive the antibody through a series of chromatography steps involving phospho- and non-phospho-peptide affinity columns for affinity purification. A highly similar content can be generated based on this original information with some rearranging.

Clonality

Polyclonal Antibody

Ensure that the storage temperature is maintained at -20 °C for a period of one year. It is important to prevent any instances of freezing and thawing cycles from occurring repeatedly throughout this time.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane. May act by activating the lipid kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of phosphorylated inositol lipids, which induces a sequential production of DAG, phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and generation of specific transport carriers to the cell surface. Under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment in newly forming focal adhesion. In osteoblast differentiation, mediates the bone morphogenetic protein 2 (BMP2)-induced nuclear export of HDAC7, which results in the inhibition of HDAC7 transcriptional repression of RUNX2. In neurons, plays an important role in neuronal polarity by regulating the biogenesis of TGN-derived dendritic vesicles, and is involved in the maintenance of dendritic arborization and Golgi structure in hippocampal cells. May potentiate mitogenesis induced by the neuropeptide bombesin or vasopressin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. Plays an important role in the proliferative response induced by low calcium in keratinocytes, through sustained activation of MAPK1/3 (ERK1/2) pathway. Downstream of novel PKC signaling, plays a role in cardiac hypertrophy by phosphorylating HDAC5, which in turn triggers XPO1/CRM1-dependent nuclear export of HDAC5, MEF2A transcriptional activation and induction of downstream target genes that promote myocyte hypertrophy and pathological cardiac remodeling. Mediates cardiac troponin I (TNNI3) phosphorylation at the PKA sites, which results in reduced myofilament calcium sensitivity, and accelerated crossbridge cycling kinetics. The PRKD1-HDAC5 pathway is also involved in angiogenesis by mediating VEGFA-induced specific subset of gene expression, cell migration, and tube formation. In response to VEGFA, is necessary and required for HDAC7 phosphorylation which induces HDAC7 nuclear export and endothelial cell proliferation and migration. During apoptosis induced by cytarabine and other genotoxic agents, PRKD1 is cleaved by caspase-3 at Asp-378, resulting in activation of its kinase function and increased sensitivity of cells to the cytotoxic effects of genotoxic agents. In epithelial cells, is required for transducing flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, which contributes to MAPK14/p38 activation and production of inflammatory cytokines. May play a role in inflammatory response by mediating activation of NF-kappa-B. May be involved in pain transmission by directly modulating TRPV1 receptor. Plays a role in activated KRAS-mediated stabilization of ZNF304 in colorectal cancer (CRC) cells (PubMed:24623306). Regulates nuclear translocation of transcription factor TFEB in macrophages upon live S.enterica infection (By similarity).

Tissue specificity

Up-regulated by the intestine-specific transcription factor CDX1 in a activated KRAS-dependent manner in colorectal cancer (CRC) cells (PubMed:24623306).

Celluar localization

Cytosol;Golgi apparatus;Plasma Membrane;

Q15139/Q9BZL6/O94806