Phospho-IGF1R (Tyr1165/Tyr1166) Rabbit Polyclonal Antibody#abs130751

abs130751-50ug

1-2 Weeks

201

abs130751-100ug

1-2 Weeks

301

Description

The high-affinity receptor for insulin-like growth factor is associated with tyrosine kinase activity. Its crucial role in transformation events is well-established, as it participates in several key cellular processes. Via the generation of alpha and beta subunits, the precursor to the insulin-like growth factor I receptor undergoes cleavage. The receptor is essential in the proper functioning of various biological systems, and its dysregulation can cause a multitude of issues.

Other names

The CD221 antigen, also known as IGF 1 receptor or IGF-I receptor, plays a crucial role in mediating cellular responses to insulin-like growth factors. It is encoded by the Igf1r gene and exists in both membrane-bound and soluble forms. In addition to its role in regulating cell growth and survival, the IGF 1 receptor is also involved in various diseases, including cancer, diabetes, and growth disorders. Other aliases for this protein include Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor beta chain, JTK13, and MGC18216. Additionally, there are two variants of soluble IGF1R, variant 1 and variant 2, that have been identified.

Source

Rabbit

Specificity

The Phospho-IGF1R (Tyr1165/Tyr1166) Antibody can detect the presence of IGF1R, but only when it is phosphorylated at Tyrosine 1165/Tyrosine 1166. This means that this antibody is highly specific and targeted, as it detects the precise state of the protein that is relevant to its biological function. By using this antibody, researchers can uncover important insights into the signaling pathways controlled by IGF1R, and gain a deeper understanding of its role in various cellular processes. Overall, the Phospho-IGF1R (Tyr1165/Tyr1166) Antibody is a powerful tool for investigating the complex world of protein regulation.

Predictive reaction species

Chicken;Rabbit;Xenopus;Dog;Bovine

Application

For Western Blotting, the recommended dilution range for this antibody is between 1:500 and 1:2000. When using Immunohistochemistry, the antibody should be diluted to a range of 1:50 to 1:200. For Immunofluorescence/Immunocytochemistry, the recommended dilution range is 1:100 to 1:500. If using ELISA with a peptide substrate, the antibody should be diluted to a range of 1:20000 to 1:40000. It is important to follow these guidelines to ensure accurate and reliable results in your experiments.

A peptide has been synthesized from human IGF1R, which is based around the phosphorylation site of Tyrosine 1165/Tyrosine 1166. The synthesized peptide bears a close resemblance to the original protein and serves as a useful tool for further studies and experimentation. The targeted site of phosphorylation is critical in regulating the function of IGF1R, making this synthesized peptide an essential resource for investigating its role and function in various cellular processes. The development of this synthesized peptide is a significant step towards understanding the intricate mechanisms that govern cellular processes and provides a useful tool for further research in this area.

Properties

1mg/ml

Purification

Purified rabbit serum was utilized to obtain the antibody through sequential chromatography on phospho- and non-phospho-peptide affinity columns. The affinity purification technique was employed to isolate and purify the antibody from the serum.

Clonality

Polyclonal Antibody

The recommended storage condition for this product is at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

The Rabbit IgG is stored in a solution containing phosphate buffered saline at pH 7.4, 150mM NaCl, and 0.02% sodium azide, as well as 50% glycerol, and must be kept at a temperature of -20 °C. The product is stable for up to 12 months from the date of receipt.

Target

Background

 Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.

Tissue specificity

Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.

Celluar localization

Plasma Membrane;

P08069