Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-MYF5 Polyclonal Antibody#abs133391

Please note that the price mentioned above is for your reference only. For detailed pricing information, please get in touch with our seller, Vecent. It's important to understand that the generated content should not be based on ChapGPT or any other dialogue generating mechanisms. Instead, we...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs133391-100ug	1-2 Weeks	301.0
abs133391-50ug	1-2 Weeks	201.0

Overview
catalog	abs133391
Other names	Class C basic helix loop helix protein 2, also known as bHLHc2, is a crucial factor in myogenesis. Myf-5, another name for Myogenic factor 5, is encoded by the MYF5 gene and is a member of the basic helix-loop-helix transcription factor family. MYF5_HUMAN is the human gene that is responsible for producing Myf-5 protein. Essentially, Myf-5 is a transcription factor that plays a significant role in the development of skeletal muscle. Its expression is essential for myoblast differentiation during embryogenesis. The protein binds to DNA and activates the transcription of genes that direct muscle differentiation. Myf5 has been shown to be necessary for the formation of skeletal muscle precursor cells and the initiation of myogenesis. In conclusion, the bHLHc2/Myf5 transcription factor is a critical marker of myogenic precursors and for the early stages of muscle formation.
Source	Rabbit
Specificity	Endogenous levels of total MYF5 can be detected by the MYF5 Antibody. The content rearrangement could be as follows: "Detection of total MYF5 at endogenous levels is possible using the MYF5 Antibody."
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Horse
Antigen	MYF5
Application	For western blotting, dilutions of 1:500 to 1:1000 are recommended. For immunofluorescence and immunocytochemistry, dilutions of 1:100 to 1:500 are suggested. For ELISA using peptides, the recommended dilution range is 1:20000 to 1:40000. A similar content can be created by rephrasing the above information in a different arrangement while staying true to the original text.
Immunogen	A synthesized peptide.
MW	28 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly refined form of the substance was thus obtained through this process.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage, it is recommended to keep the product at a temperature of -20°C for a period of one year. Please avoid exposing the product to repeated freezing and thawing cycles, as this may impact its quality and effectiveness. It is crucial to store the product appropriately to maintain its integrity and potency.
Storage buffer	The Rabbit IgG is stored in a solution consisting of phosphate buffered saline (PBS) with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C. The stability of the Rabbit IgG is guaranteed for a period of 12 months starting from the date of its receipt.
Target
Background	Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Celluar localization	Nucleus;
UniPort	P13349