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Rabbit Anti-ELF4 Polyclonal Antibody#abs134980

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Description

Catalog-specification	Delivery time	USD price
abs134980-100ug	1-2 Weeks	301.0
abs134980-50ug	1-2 Weeks	201.0

Overview
catalog	abs134980
Other names	Elf4, also known as E74-like factor 4 or myeloid Elf1-like factor, is a transcription factor that plays a crucial role in various cellular processes. It belongs to the ETS-related transcription factor family and is encoded by the ELF4 gene in humans. Elf4 is involved in the regulation of gene expression and has been implicated in the development and function of different cell types. The protein encoded by ELF4, also referred to as Elf-4 or MEF (Myeloid Elf-1-like factor), has been extensively studied due to its significant impact on cellular mechanisms. Its functions include controlling the activation or repression of target genes by binding to their specific regulatory regions. Elf4 is known to play a vital role in immune responses, hematopoiesis, and developmental processes. Elf4 serves as a transcriptional regulator by interacting with other proteins and forming complexes that modulate gene expression. It exhibits a high degree of similarity to Elf-1, another member of the ETS transcription factor family. The expression and activity of Elf4 are tightly regulated and can be influenced by various signaling pathways and environmental cues. The importance of Elf4 in diverse biological processes is underscored by its association with several diseases. Dysregulation of Elf4 has been implicated in cancer, immune disorders, and developmental abnormalities. Consequently, understanding the precise mechanisms by which Elf4 functions is crucial for unraveling its potential as a therapeutic target or diagnostic marker. In conclusion, Elf4 is a crucial transcription factor that plays a versatile role in regulating gene expression and cellular processes. Its intricate involvement in various biological systems highlights its significance and potential as a target for further research and therapeutic interventions.
Source	Rabbit
Specificity	The total ELF4 levels in the body can be accurately identified using the ELF4 Antibody, which is designed to specifically detect endogenous ELF4 protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	ELF4
Application	To ensure accurate results, we recommend using WB 1:500~1:1000, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. These are the optimal dilution ratios for these methods. It's important to note that when conducting WB, a higher dilution may be necessary if a weaker signal is observed. Similarly, for IF/ICC, a lower dilution may be needed if the signal is too strong. When using ELISA with a peptide substrate, a higher dilution is recommended to ensure the accuracy of the results. Overall, appropriate dilution ratios are crucial for obtaining reliable data in these assays.
Immunogen	A synthesized peptide.
MW	70 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. A highly similar content can be generated by rearranging the order of the original text information.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal preservation, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze and thaw cycles, as this may compromise its quality. It is advised to follow these guidelines in order to maintain the integrity of the product and ensure its efficacy.
Storage buffer	This rabbit IgG comes in a solution containing phosphate buffered saline at pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store it at -20°C and can be kept for up to 12 months from the date of receipt. Ensure to maintain the integrity of the original text information while generating a highly similar content.
Target
Background	Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway.
Tissue specificity	Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain.
Celluar localization	Nucleus;
UniPort	Q99607