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Rabbit Anti-CLP1 Polyclonal Antibody#abs139294

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Description

Catalog-specification	Delivery time	USD price
abs139294-100ug	1-2 Weeks	301.0
abs139294-50ug	1-2 Weeks	201.0

Overview
catalog	abs139294
Other names	CLP1, also known as Cleavage and Polyadenylation Factor I Subunit 1, is an important ATP/GTP binding protein that plays a crucial role in pre-mRNA cleavage and polyadenylation processes. As a subunit of the cleavage and polyadenylation factor I complex, CLP1 is responsible for catalyzing the phosphorylation of the 5' end of RNA molecules to facilitate their subsequent polyadenylation and processing. Homologous to the yeast CFIA subunit Clp1p, CLP1 is highly conserved across different species and is essential for the proper functioning of the mRNA processing machinery. It has been identified as a key factor in regulating gene expression and is involved in a variety of cellular processes, including cell differentiation, proliferation, and apoptosis. With its vital role in pre-mRNA processing, CLP1 has become an increasingly important target for therapeutic intervention in a range of diseases. In particular, dysregulation of CLP1 has been implicated in various cancers, highlighting the potential of CLP1 inhibition as a new avenue for cancer treatment. In summary, CLP1 is a crucial component of the mRNA processing machinery that plays an important role in gene expression regulation and is a promising therapeutic target for various diseases.
Source	Rabbit
Specificity	The antibody CLP1 can identify total levels of CLP1 that are endogenous. This means that the antibody is able to detect the natural amounts of CLP1 produced by the body without any external interference. By using this antibody, researchers can accurately measure the levels of this protein within a sample.
Species Reactivity	Human;Mouse;Rat
Antigen	CLP1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human CLP1.
MW	48 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was done through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, keep the product frozen at a temperature of -20 °C for a period of one year. It is important to avoid any cycles of repeated freezing and thawing in order to maintain the product's quality.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Celluar localization	Cytosol;Nucleus;
UniPort	Q92989