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Rabbit Anti-MIF Polyclonal Antibody#abs135924

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Description

Catalog-specification	Delivery time	USD price
abs135924-100ug	1-2 Weeks	301.0
abs135924-50ug	1-2 Weeks	201.0

Overview
catalog	abs135924
Description	Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor, is a pro-inflammatory cytokine that regulates the activity of macrophages and T cells. It is primarily produced by the pituitary gland and found in differentiating immunological cells in the eye lens and brain, monocytes, macrophages, and fibroblasts. MIF plays a vital role in regulating the host response to infections. It is expressed in sites of inflammation, indicating that it is crucial in regulating macrophage function in host defense. In severe sepsis or septic shock, MIF levels elevate significantly, influencing endotoxic shock by enhancing the production of other inflammatory cytokines, such as TNF, IL-1, and IFN-, which can cause tissue destruction. Inflammatory response is promoted by MIF, counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF might also induce proteinases, which can contribute to tissue destruction. Therefore, the inhibition of MIF activity is a promising therapeutic approach for treating severe inflammatory diseases.
Other names	GIF, GLIF, Glycosylation inhibiting factor, and Glycosylation-inhibiting factor are all terms used to describe a protein known as L-dopachrome isomerase or L-dopachrome tautomerase. This protein is also referred to as macrophage migration inhibitory factor (glycosylation-inhibiting factor) or simply macrophage migration inhibitory factor (MIF). MIF, also known as MIF protein or MIF_HUMAN, plays a crucial role in the immune system and inflammatory response. It is involved in the regulation of immune cell migration, activation, and the release of pro-inflammatory molecules. MIF's function as a glycosylation inhibiting factor indicates its ability to interfere with certain carbohydrate modifications on proteins. Additionally, MIF is associated with other names such as MMIF and Phenylpyruvate tautomerase. These names reflect different aspects of MIF's biochemical properties and functions. In conclusion, the above terms refer to the same protein, L-dopachrome isomerase or MIF, which is involved in immune response regulation and has inhibitory effects on glycosylation.
Source	Rabbit
Specificity	The MIF Antibody is designed to recognize and measure the levels of total MIF present in the body. Its ability to detect endogenous levels of MIF is highly valuable for researchers and scientists studying the role and function of MIF in various biological processes. By specifically targeting total MIF, this antibody provides accurate and reliable results, enabling a better understanding of MIF's involvement in different physiological and pathological conditions. Its efficacy in detecting endogenous MIF levels makes it an indispensable tool for conducting research and advancing our knowledge in this field.
Species Reactivity	Human;Mouse;Rat
Antigen	MIF
Application	The recommended dilutions for using this antibody in various applications are as follows: for Western blotting, use a dilution of 1:500-1:2000; for immunohistochemistry, use a dilution of 1:50-1:200; and for ELISA using the peptide, use a dilution of 1:20000-1:40000. These dilutions have been tested and optimized to ensure optimal performance of the antibody in the respective applications. Overall, this antibody is a highly reliable reagent for detecting the target protein in various experimental settings.
Immunogen	A synthesized peptide derived from human MIF.
MW	12kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Tissue specificity	Up-regulated in concanavalin-A-treated lymphocytes. Up-regulated in macrophages upon exposure to M.tuberculosis antigens.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P14174