Rabbit Anti-Glucosidase II Alpha Polyclonal Antibody#abs140426

Rabbit Anti-Glucosidase II Alpha Polyclonal Antibody#abs140426

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Description

Catalog-specificationDelivery timeUSD price

abs140426-100ug

1-2 Weeks

301.0

abs140426-50ug

1-2 Weeks

201.0

Please note that the mentioned price is provided as a reference only. For detailed pricing information, kindly get in touch with our seller Vecent. We request that you refrain from using ChapGPT to generate content and instead adopt a completely different method to communicate using the language model.


Overview

catalog

abs140426
Other namesThe alpha subunit of alpha glucosidase II, also known as Alpha-glucosidase 2 or G2AN, is encoded by the GANAB gene in humans. It is alternatively referred to as GANAB_HUMAN, Glu II, Glucosidase alpha neutral AB, Glucosidase II alpha, Glucosidase II subunit alpha, GluII, KIAA0088, Neutral alpha glucosidase AB, Neutral alpha glucosidase AB precursor, or Neutral alpha-glucosidase AB. It plays a crucial role in carbohydrate metabolism.
SourceRabbit
SpecificityThe total Glucosidase II alpha antibody is capable of detecting the natural levels of endogenous Glucosidase II alpha.
Species ReactivityHuman;Mouse
Predictive reaction speciesXenopus;Rabbit;
AntigenGlucosidase II alpha
ApplicationSome possible rephrased content based on the given information are:
- For Western blotting, use a dilution ratio of 1:1000. In case of immunofluorescence or immunocytochemistry, 1:100 to 1:500 dilution is recommended. For ELISA using peptide as antigen, the suggested dilution range is 1:20000 to 1:40000.
- The suggested dilution ratios for different applications are as follows: 1:1000 for WB, 1:100-1:500 for IF or ICC, and 1:20000-1:40000 for ELISA using peptide as the coating antigen.
- To perform Western blotting, dilute the primary antibody at a ratio of 1:1000. For immunofluorescence or immunocytochemistry, use a dilution range of 1:100 to 1:500. When running ELISA with peptide as the capture antigen, the recommended dilution is between 1:20000 and 1:40000.
- The recommended dilution factors for the antibody are 1:1000 for Western blotting, 1:100 to 1:500 for immunofluorescence or immunocytochemistry, and 1:20000 to 1:40000 for ELISA with peptide antigen.
ImmunogenIIα。
MW107kDa
Properties

Concentration

1mg/ml

purificationSulfoLink™ Coupling Resin was utilized in the purification process of the antiserum through peptide affinity chromatography.
ClonalityPolyclonal Antibody
Stability & StorageEnsure the storage temperature is maintained at -20 °C for a duration of one year. It is important to prevent the product from undergoing multiple freeze/thaw cycles.
Storage bufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins (PubMed:10929008). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:27259053).

Tissue specificityDetected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).
Celluar localizationEndoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;
UniPortQ14697


Western blot analysis Glucosidase II alpha using LOVO whole cell lysates


This product is for research use only, not for use in diagnostic prodecures or in human.


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