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Rabbit Anti-CBS Polyclonal Antibody#abs135930

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, we kindly request you to contact our seller, Vecent. Western blot analysis of extracts of mouse kidneytissue, using CBS antibody. This product is for research use only, not for use...

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Description

Catalog-specification	Delivery time	USD price
abs135930-100ug	1-2 Weeks	301.0
abs135930-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, we kindly request you to contact our seller, Vecent.

Overview
catalog	abs135930
Description	Cystathionine beta-synthase (CBS) is encoded by the CBS gene and plays a crucial role in the initial irreversible step of transsulfuration. The CBS enzyme consists of four identical subunits weighing 63-kD each and requires pyridoxal phosphate and heme to function effectively (PMID:11230183). CBS protein is primarily found in various regions of the brain, with a notable presence in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons (PMID:12588964). It is important to note that this antibody specifically targets CBS, ensuring accurate detection of the protein.
Other names	Cystathionine beta-synthase, also known as CBS, is a protein that plays a crucial role in the metabolism of homocysteine. This enzyme is responsible for the conversion of homocysteine to cystathionine, which is then converted to the amino acid methionine. CBS is a highly conserved enzyme found in many organisms, including humans, and is essential for proper metabolic function. Beta-thionase, another name for CBS, is also involved in the synthesis of methylcysteine and serine sulfhydrase. CBS deficiency is linked to homocystinuria, a genetic disorder that causes high levels of homocysteine in the blood and can lead to serious health problems, such as cardiovascular disease and neurological damage. The CBS gene is located on chromosome 21 and mutations in this gene can lead to reduced enzymatic activity or complete loss of function, resulting in homocystinuria. Treatment for this disorder involves managing symptoms and administering vitamin supplementation to lower homocysteine levels. Overall, CBS is an essential enzyme that plays a critical role in maintaining proper metabolic function and preventing serious health problems associated with homocystinuria.
Source	Rabbit
Specificity	The endogenous levels of total CBS can be accurately detected using the CBS Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;
Antigen	CBS
Application	The recommended dilution range for using the antibody in Western Blot is 1:500 to 1:2000, while for Immunohistochemistry 1:50 to 1:200 is recommended. For ELISA (peptide), the recommended dilution range is between 1:20000 and 1:40000. It is important to note that the dilution range will vary depending on the target and the sample being used. By carefully selecting the dilution, the user can achieve optimal results with this antibody.
Immunogen	A synthesized peptide derived from human CBS.
MW	61kDa
Properties
Concentration	1mg/ml
purification	Purification of the antiserum was carried out through peptide affinity chromatography utilizing SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:23981774, PubMed:20506325, PubMed:23974653). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).
Tissue specificity	In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
Celluar localization	Cytosol;Nucleus;
UniPort	P35520