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Rabbit Anti-RAC2 Polyclonal Antibody#abs135793

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Description

Catalog-specification	Delivery time	USD price
abs135793-100ug	1-2 Weeks	301.0
abs135793-50ug	1-2 Weeks	201.0

Overview
catalog	abs135793
Description	Rac and Cdc42 belong to the Rho-GTPase family and are important for various cellular processes, including cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development. In mammals, Rac is present in three isoforms, namely Rac1, Rac2, and Rac3, which exhibit high sequence homology. While Rac1 and Cdc42 are widely expressed, Rac2 is predominantly expressed in cells of hematopoietic origin, and Rac3 is abundantly expressed in the brain and other tissues. The activity of Rac/Cdc42 is stimulated by GTP binding, and hydrolysis of GTP to GDP renders the protein inactive. The intrinsic GTPase activity of Rac/Cdc42 is aided by GAPs, while GEFs facilitate the exchange of GDP for GTP. The binding of RhoGDI to Rac/Cdc42 prevents its activation by keeping it in the inactive GDP-bound state. Overall, Rac and Cdc42 are critical regulators of various cellular processes and their activity is tightly controlled by various regulatory mechanisms, such as GTP hydrolysis and RhoGDI-mediated inhibition.
Other names	Rac2, also known as p21-Rac2 or Ras related C3 botulinum toxin substrate 2, is a small GTP binding protein belonging to the Rho family. It plays an important role in various cellular processes, including cytoskeletal reorganization, cell migration, and phagocytosis. Rac2 is encoded by the RAC2 gene and is highly similar to Rac1, another member of the Rho family. In addition to Rac2, there is also Ras related C3 botulinum toxin substrate 3 (Rac3), which is another small GTP binding protein of the Rho family. Rac3 is encoded by the RAC3 gene and shares significant sequence similarity with both Rac1 and Rac2. Overall, the Rho family of small GTP binding proteins, including Rac2 and Rac3, are important regulators of cellular processes and are implicated in various diseases, such as cancer and immune disorders. Understanding their function and regulation can have important implications for developing targeted therapies.
Source	Rabbit
Specificity	The RAC2 Antibody is capable of recognizing total RAC2 levels that naturally occur within the body. This information can be rearranged to create a similarly meaningful sentence.
Species Reactivity	Human;Mouse;Rat
Antigen	RAC2
Application	The recommended dilutions for antibody applications are as follows: 1:500-1:2000 for Western blotting, 1:50-1:200 for immunohistochemistry, and 1:20000-1:40000 for ELISAs using peptide substrates. It is important to adhere to the specified dilutions in order to achieve optimal results.
Immunogen	A synthesized peptide derived from human RAC2.
MW	21kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Tissue specificity	Hematopoietic specific.
Posttranslational modification	(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P15153