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Rabbit Anti-RALBP1 Polyclonal Antibody#abs135794

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Description

Catalog-specification	Delivery time	USD price
abs135794-100ug	1-2 Weeks	301.0
abs135794-50ug	1-2 Weeks	201.0

Overview
catalog	abs135794
Description	RalA binding protein 1 (RalBP1 or RLIP76) was initially discovered as a downstream effector of RalA in Ral-Ras signaling, forming a complex with GTP-RalA and the endocytosis protein REPS2 (POB1) through its carboxy-terminal Ral binding domain. With its centrally located Rho-GAP domain, RalBP1 also interacts with Cdc42 and exhibits intrinsic GTPase activating function. This protein complex, consisting of RalBP1/POB1/RalA, plays a crucial role in regulating the endocytosis of membrane receptors. Moreover, RalBP1 serves as a transporter, utilizing ATP to facilitate the transport of various xenobiotics such as glutathione conjugates and certain chemotherapeutic agents. Its transporter activity is associated with detoxification, drug resistance, and stress response mechanisms. Significantly, increased expression of RalBP1 is observed in specific cancer types, and inhibiting RalBP1 results in the regression of cancer xenografts. Therefore, targeting RalBP1 shows great promise as a potential therapeutic strategy for cancer treatment.
Other names	The protein RLIP1, also known as Ral-interacting protein 1 or RalBP1, is a 76-kDa protein that is responsible for ATPase activity of Dinitrophenyl S-glutathione. RLIP1 is also capable of binding to the RalA protein. This protein, which is encoded by the gene RBP1_HUMAN, is commonly referred to as RIP1 or RLIP76.
Source	Rabbit
Specificity	The RALBP1 Antibody is capable of detecting the complete range of RALBP1 present within the body. This means that it can identify the naturally occurring levels of RALBP1 without any external interference. The antibody is specifically designed to recognize and bind to the RALBP1 protein, allowing for accurate and reliable detection. Overall, this antibody is an excellent tool for researchers interested in studying RALBP1 and its various functions within the body.
Species Reactivity	Human;Mouse;Rat
Antigen	RALBP1
Application	The recommended dilutions for WB are 1:500-1:2000, while for IHC, they are 1:50-1:200. As for ELISA (peptide), a dilution of 1:20000-1:40000 is suggested. It is important to note that these dilutions are based on the original text information and should be followed to ensure reliable results.
Immunogen	A synthesized peptide derived from human RALBP1.
MW	76kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
Tissue specificity	Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
Celluar localization	Cytosol;
UniPort	Q15311