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Rabbit Anti-TCF4 Polyclonal Antibody#abs135795

Description

Catalog-specification	Delivery time	USD price
abs135795-100ug	1-2 Weeks	301.0
abs135795-50ug	1-2 Weeks	201.0

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Overview
catalog	abs135795
Description	LEF1 and TCF belong to the HMG DNA binding protein family of transcription factors, which also includes TCF1, TCF3, and TCF4. Initially, LEF1 and TCF1 were recognized as crucial factors regulating early lymphoid development. Both proteins play a crucial role downstream in the Wnt signaling pathway and bind to Wnt response elements, creating nucleation sites for β-catenin. Upon activation of Wnt signaling, β-catenin moves to the nucleus to boost the transcription of target genes. The expression levels of LEF1 and TCF are dynamic during development, and the Wnt signaling pathway's aberrant activation is implicated in various cancers, such as colon cancer.
Other names	bHLHb19, also known as Class B basic helix-loop-helix protein 19 or E2 2, is a crucial protein involved in various biological processes. It is also referred to as FECD3, Immunoglobulin transcription factor 2, ITF 2, ITF-2, ITF2_HUMAN, MGC149723, MGC149724, PTHS, SEF 2, SEF-2, SEF2 1, SEF2 1A, SEF2 1B, SEF2 1D, SEF2, SL3 3 enhancer factor 2, SL3-3 enhancer factor 2, TCF 4, TCF-4, TCF4, Transcription factor 4, Transcription factor 4, isoform C, Transcription factor 4, isoform D, Transcription factor 4, isoform E, Transcription factor 4, isoform L, and Transcription factor 4, isoform M, Transcription factor 4, isoform R. The bHLHb19 protein plays a pivotal role in regulating gene expression and control of cell differentiation. It is involved in the formation of specific tissues and organs during development. Research indicates that defects in the bHLHb19 gene can lead to certain developmental disorders. Furthermore, bHLHb19 has been associated with immunoglobulin transcription regulation and is crucial for the immune system's proper functioning. It acts as a transcription factor, modulating the expression of genes involved in immune responses. Additionally, bHLHb19 has been linked to the pathogenesis of psychiatric and neurological disorders. Studies have suggested that variations in the bHLHb19 gene may contribute to the development of certain mental illnesses. Overall, bHLHb19, with its multiple aliases, represents a significant protein in various biological functions, ranging from embryonic development to immune system regulation and potential implications in psychiatric disorders. Its comprehensive understanding and further research can pave the way for novel therapeutic approaches and targeted interventions.
Source	Rabbit
Specificity	The endogenous levels of total TCF4 can be detected using the TCF4 Antibody. We have developed this antibody to specifically target and measure the natural levels of TCF4 in biological samples. By utilizing this antibody, researchers can accurately assess the amount of TCF4 present in their experimental systems. This information is crucial for understanding the role of TCF4 in various cellular processes and can provide valuable insights into its involvement in disease mechanisms.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Sheep;Dog;Horse;Bovine
Antigen	TCF4
Application	For Western Blotting, dilutions of 1:500 to 1:2000 are recommended, whereas for Immunohistochemistry, a dilution range of 1:50 to 1:200 should be used. For ELISA assays using a peptide substrate, the recommended dilution range is 1:20000 to 1:40000. These dilution guidelines are crucial in ensuring accurate and reliable results in these experimental techniques. Therefore, it is important to follow these recommended ranges while conducting these assays in order to obtain accurate and reproducible data.
Immunogen	A synthesized peptide derived from human TCF4.
MW	71kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Tissue specificity	Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
Celluar localization	Nucleus;
UniPort	P15884