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Rabbit Anti-SH2D1A Polyclonal Antibody#abs135797

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Description

Catalog-specification	Delivery time	USD price
abs135797-100ug	1-2 Weeks	301.0
abs135797-50ug	1-2 Weeks	201.0

Overview
catalog	abs135797
Description	The proteins SH2D1A and SH2D1B play significant roles in signal transduction through the SLAM family receptors, as they possess a single SH2-domain and act as adaptor proteins. Mutations in SH2D1A, also referred to as SAP or SLAM-associated protein, are frequently observed in patients with X-linked lymphoproliferative disease (Duncan's disease). This disorder is characterized by an extreme vulnerability to the Epstein-Barr virus, and over 50 different SH2D1A mutations have been identified thus far. In humans, SH2D1B (also known as EAT-2 or Ewing's sarcoma's/FLI1-activated transcript 2) is a single gene, while it exists as a pair of duplicated genes, EAT-2A and EAT-2B, with identical genomic organization in mice and rats.
Other names	This is information about a protein called SH2 domain-containing protein 1A, also known as Duncan disease SH2-protein, SLAM associated protein, or T cell signal transduction molecule SAP. It is associated with a genetic disease called X-linked lymphoproliferative syndrome, or XLP, which affects the immune system and can lead to severe complications such as organ failure or cancer. The protein plays a role in regulating the signaling pathways that control immune cell proliferation and activation, particularly in response to viral infections. Mutations in the gene that codes for this protein can disrupt these pathways and lead to XLP. This condition is rare, but can be life-threatening if not properly managed. Therefore, early diagnosis and treatment are crucial for XLP patients.
Source	Rabbit
Specificity	The detection of total SH2D1A at endogenous levels can be achieved with the use of the SH2D1A Antibody. To rearrange the content while maintaining its essence, one can say: "By utilizing the SH2D1A Antibody, it becomes possible to detect the overall concentration of SH2D1A present naturally within the system."
Species Reactivity	Human;Mouse;Rat
Antigen	SH2D1A
Application	The recommended dilutions for WB testing range from 1:500 to 1:2000, while for IHC it is 1:50 to 1:200. ELISA testing using peptides require a dilution of 1:20000 to 1:40000 for optimal results. These dilution ranges are crucial to obtain accurate and reliable test results. It is important to follow the recommended guidelines to ensure the best possible outcome. Testing with inappropriate dilution can lead to false positive or negative results, which can affect the accuracy of the test. Therefore, it is recommended that appropriate dilutions are selected for each test to ensure optimal results.
Immunogen	A synthesized peptide derived from human SH2D1A.
MW	14kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed:11806999). However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1 (PubMed:12458214). Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells (By similarity). May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.
Tissue specificity	Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T-lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis.
Celluar localization	Cytosol;
UniPort	O60880