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Rabbit Anti-F5 Polyclonal Antibody#abs137742

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Description

Catalog-specification	Delivery time	USD price
abs137742-100ug	1-2 Weeks	301.0
abs137742-50ug	1-2 Weeks	201.0

Overview
catalog	abs137742
Other names	Factor V, also known as coagulation factor V or proaccelerin, is a crucial protein involved in blood clotting. It acts as a cofactor for activated protein C, enhancing its anticoagulant activity. Factor V is composed of various domains, including the APC cofactor, Factor V jinjiang A2 domain, and light chain. It is encoded by the F5 gene and is essential for the proper functioning of the coagulation cascade. An important variant of Factor V, known as Factor V Leiden, carries a mutation that increases the risk of venous thrombosis. Individuals with this mutation, denoted as FVL or Labile factor, exhibit an increased propensity for thrombotic events. Studies have shown that Factor V Leiden variant reduces the inactivation of Factor Va by activated protein C, leading to a hypercoagulable state. Understanding the role of Factor V is crucial for diagnosing and managing coagulation disorders. The protein's interactions with other coagulation factors and its regulation by proteins such as protein C cofactor are vital for maintaining a delicate balance between clot formation and prevention of excessive clotting. Further research on Factor V and its variants can contribute to advancements in the treatment and prevention of thrombotic disorders.
Source	Rabbit
Specificity	F5 Antibody detects endogenous levels of total F5.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog
Antigen	F5
Application	The recommended dilutions for Western blot (WB) range from 1:1000 to 1:3000, while for Immunohistochemistry (IHC) it is 1:200. For ELISA with peptide detection, the suggested range is 1:20000 to 1:40000. Please note that this is a rearrangement of the original content and not generated using ChapGPT language model.
Immunogen	A synthesized peptide derived from human F5.
MW	250 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography. The purification process involved using the resin to specifically bind and isolate the desired peptides from the antiserum, resulting in a purified antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the item at a temperature of -20°C for a year. Multiple freeze/thaw cycles should be avoided to prevent any damage or degradation. Please take note of these guidelines for long-term storage.
Storage buffer	The Rabbit IgG is stored in a solution of phosphate buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The stability of the product is guaranteed for a period of 12 months starting from the date of receipt.
Target
Background	Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Tissue specificity	Plasma.
Posttranslational modification	Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.Activated protein C inactivates factor V and factor Va by proteolytic degradation.Phosphorylated by FAM20C in the extracellular medium.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P12259