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Rabbit Anti-S6K Polyclonal Antibody#abs133426

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. Western blot analysis of extracts from COLO205 cells and Jurkat cells, using S6K antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs133426-100ug	1-2 Weeks	301.0
abs133426-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.

Overview
catalog	abs133426
Other names	The 70 kDa ribosomal protein S6 kinase 1, also known as RPS6KB1, plays a vital role in cell growth and growth factor signaling. This kinase is encoded by the p70 S6 kinase gene and exists in two isoforms, alpha and beta. It facilitates cellular division by phosphorylating the ribosomal protein S6 and other targets in the mTOR pathway. The p70 S6 kinase alpha and beta isoforms, also known as p70 alpha and p70 beta 1, respectively, are critical for regulating various cellular processes such as translation, transcription, and metabolism. They both are activated by extracellular signals and are involved in numerous signaling pathways, including the MAPK/ERK, PI3K/Akt, and Wnt pathways. Other aliases for this kinase enzyme include KS6B1_HUMAN, P70S6K, P70S6K1, P70 S6 kinase, Ribosomal protein S6 kinase beta 1, and S6K1. It has also been referred to as STK14A or Serine/threonine kinase 14 alpha. In summary, the p70 S6 kinase plays a crucial role in regulating cell growth factors, metabolism, and translation by phosphorylating key targets in the mTOR pathway. Its isoforms, p70 alpha and p70 beta 1, are involved in multiple signaling pathways and are essential for various cellular processes.
Source	Rabbit
Specificity	The S6K Antibody is capable of identifying the complete S6K present within the body. The antibody recognizes endogenous levels of the protein and can be used to detect its total presence. Simply put, it is a useful tool for researchers looking to study S6K expression.
Species Reactivity	Human;Mouse;Rat
Antigen	S6K
Application	For western blotting, we recommend using a dilution range of 1:500 to 1:1000, while for immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended. If you're using immunofluorescence or immunocytochemistry, we suggest a dilution range of 1:100 to 1:500. For ELISA using peptide, we recommend using a dilution range of 1:20000 to 1:40000. It's important to note that optimized dilutions may vary depending on the specific experiment or sample being used.
Immunogen	A synthesized peptide.
MW	70 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the purification of the antiserum through peptide affinity chromatography. Let's generate a highly similar content by rearranging the above information while ensuring that it is based on the original text.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a year. It is important to avoid subjecting it to repeated freezing and thawing cycles. This will ensure that the product remains stable and effective for use. Therefore, it is best to take precautions to keep the product in optimal condition for extended periods of time.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.
Tissue specificity	Widely expressed.
Posttranslational modification	Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	P23443