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Rabbit Anti-RFWD2 Polyclonal Antibody#abs141102

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly ask you to contact our seller Vecent. Western blot analysis of extracts from LOVO cells, using RFWD2 antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs141102-100ug	1-2 Weeks	301.0
abs141102-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly ask you to contact our seller Vecent.

Overview
catalog	abs141102
Other names	Homolog of constitutive photomorphogenesis protein 1, also known as COP, is a protein encoded by the RING finger and WD repeat domain 2 (RFWD2) gene. The gene is responsible for the E3 ubiquitin-protein ligase RFWD2, an important protein involved in the regulation of various cellular processes. The human COP1 protein, also referred to as hCOP1, plays an essential role in development and growth as it regulates the processes that control cell division, differentiation, and apoptosis. RFWD2, with its RING finger and WD repeat domains, is an essential component of the ubiquitin-proteasome system, which is responsible for degrading and eliminating misfolded or damaged proteins from the cell. In addition to its ubiquitin-ligase activity, RFWD2 also plays a role in transcriptional regulation, DNA damage repair, and in the control of the circadian clock. Overall, the RING finger and WD repeat domain protein 2 is a critical player in maintaining cellular homeostasis and ensuring the proper function of a variety of cellular processes.
Source	Rabbit
Specificity	The RFWD2 Antibody is capable of detecting the overall levels of RFWD2 that are present in the body. This antibody can identify the endogenous RFWD2, indicating that it is naturally produced within the body. To rephrase this information, one may state that the RFWD2 Antibody can detect the total amount of endogenous RFWD2 levels that are present in the body.
Species Reactivity	Human;Mouse
Predictive reaction species	Bovine
Antigen	RFWD2
Application	We have WB dilutions ranging from 1:500 to 1:1000, IF/ICC dilutions ranging from 1:100 to 1:500, and ELISA (peptide) dilutions ranging from 1:20000 to 1:40000. It is important to note that the generated content is not generated using the method employed by ChapGPT, but rather through an alternative language model.
Immunogen	A synthesized peptide.
MW	80 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to store at a temperature of -20 °C for a duration of one year to maintain the quality. To preserve the integrity, it is important to avoid subjecting the product to repeated cycles of freezing and thawing.
Storage buffer	The storage condition for Rabbit IgG is -20 °C with a formulation of phosphate buffered saline (pH 7.4), 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It can be stored for up to 12 months from the date of receipt.
Target
Background	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).
Tissue specificity	Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
Posttranslational modification	Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination.
Celluar localization	Cytosol;Golgi apparatus;Nucleus;
UniPort	Q8NHY2