Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-TOPRS Polyclonal Antibody#abs132854

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller Vecent. We recommend reaching out to Vecent to obtain accurate and specific pricing details. Western blot analysis of TOPRS expression in HEK293 cells ,The...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs132854-100ug	1-2 Weeks	301.0
abs132854-50ug	1-2 Weeks	201.0

Overview
catalog	abs132854
Other names	Topors, also known as E3 ubiquitin protein ligase Topors, is a multifunctional protein involved in various cellular processes. It is encoded by the AW105885 gene and is characterized by its ability to interact with different binding partners. One of its important functions is as an E3 ubiquitin-protein ligase, which means it helps in attaching ubiquitin molecules to target proteins. This process plays a crucial role in protein degradation and regulation. Topors has been shown to have specific E3 ligase activity towards several proteins, including p53 and SUMO1. Another role of Topors is as a p53 binding protein. It interacts with the tumor suppressor protein p53, which is well-known for its critical role in regulating cell cycle progression and apoptosis. Through this interaction, Topors can modulate p53 activity and influence its downstream signaling pathways. Topors is also associated with retinitis pigmentosa 31 (RP31), a genetic disorder affecting the retina. Mutations in the Topors gene have been linked to the development of RP31, suggesting its involvement in maintaining retinal function. Furthermore, Topors has been shown to bind to topoisomerase I, an enzyme involved in DNA replication and repair. This interaction suggests a potential role for Topors in regulating topoisomerase I activity and genomic stability. In summary, Topors is a versatile protein with multiple functions. It acts as an E3 ubiquitin-protein ligase, a p53 binding protein, and a binding partner for topoisomerase I. Its involvement in various cellular processes highlights its significance in maintaining proper cellular function and suggests its potential as a therapeutic target in certain diseases.
Source	Rabbit
Specificity	The presence of endogenous levels of total TOPRS can be detected using TOPRS antibody. When using this antibody, it is possible to accurately assess the amount of TOPRS in a sample.
Species Reactivity	Human;Mouse
Predictive reaction species	Bovine;Rabbit;Sheep
Antigen	TOPRS
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was employed in the purification of the antiserum. A highly similar content can be generated by arranging the aforementioned information in a different manner.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.
Tissue specificity	Expressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.
Posttranslational modification	Phosphorylation at Ser-98 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity. Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.Sumoylated.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	Q9NS56