Phospho-E2F1 (Thr433) Rabbit Polyclonal Antibody#abs131002

abs131002-50ug

1-2 Weeks

201

abs131002-100ug

1-2 Weeks

301

Description

This particular gene produces a protein that belongs to the E2F group of transcription factors. The E2F cluster is essential in regulating the cell cycle and the activity of tumor suppressor proteins, as well as being a prime target for small DNA tumor viruses' transforming proteins.

Other names

E2F1, also known as Dmel\CG6376 or Dmel_CG6376, is a transcription factor that plays a crucial role in gene regulation. It is involved in various biological processes such as cell cycle progression and apoptosis. E2F1 is also associated with retinoblastoma-binding protein 3 (RBP3) and is a key player in the RB-E2F pathway. The protein is encoded by the E2F1 gene and is conserved across different species, including humans (E2F1_HUMAN). It has multiple isoforms, including E2f-PA, E2f-PB, and E2f-PC. The E2F1 protein binds to the E2-promoter region and acts as a transcriptional activator or repressor, depending on the target gene. Its interaction with other proteins, such as the PRB-binding protein E2F-1 and RBAP-1, further modulates gene expression. Additionally, E2F1 is associated with the E(Sev-CycE)3A and E(var)3-93E genomic loci. The KIAA4009 gene is another name for E2F1, and it is located on chromosome 3 in Drosophila melanogaster. The Evar(3)164 and l(3)07172 are alternative names or identifiers for E2F1 in Drosophila. Overall, E2F1 is an essential transcription factor involved in regulating key cellular processes.

Source

Rabbit

Specificity

The Phospho-E2F1 (Thr433) Antibody is designed to detect the presence of E2F1 in the body only when it is phosphorylated at Threonine 433. This means that the antibody specifically targets this particular phosphorylation, allowing researchers to identify E2F1 proteins that are activated in the body. By using this antibody, scientists can better understand the role of E2F1 in various processes and diseases, which could lead to new treatments and therapies. Overall, this antibody offers a valuable tool for studying the complex mechanisms of protein regulation in the body.

Predictive reaction species

Pig;Rabbit;Bovine;Horse;

Application

，：WB 1:500-1:2000， IF/ICC 1:100-1:500， ELISA(peptide) 1:20000-1:40000。。，Western Blotting（WB），1:500-1:2000；（IF/ICC），1:100-1:500；（ELISA），1:20000-1:40000。。。

E2F1，433。，，。

Properties

1mg/ml

Purification

This particular antibody was obtained from rabbit serum that was purified using sequential chromatography techniques on both phospho- and non-phospho-peptide affinity columns. Through this process, a highly purified sample was obtained that is similar to the original serum from which it was derived.

Clonality

Polyclonal Antibody

To ensure optimal storage, it is recommended that the product be kept at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may compromise its quality. Please handle with care to ensure the longevity and efficacy of the product.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline at pH 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store at -20 °C, and has a shelf life of 12 months from date of purchase. For optimum results, handle with care and follow proper laboratory protocols. This highly purified IgG is ideal for use in a variety of applications including immunoassays, immunoprecipitation, and western blotting. For any questions regarding usage and handling, do not hesitate to contact our support team for assistance.

Target

Background

 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).

Celluar localization

Cytoskeleton;Mitochondrion;Nucleus;

Q01094