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Rabbit Anti-S100B Polyclonal Antibody#abs135636

Please note that the price mentioned above is for reference purposes only. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of A375 cell lysate using S100B antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs135636-100ug	1-2 Weeks	301.0
abs135636-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for reference purposes only. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs135636
Description	S100 proteins, despite their simple structure and small size, play a crucial role in regulating several cellular processes. These proteins, which range from 8-12 kDa, are involved in cell growth, cell motility, cell cycle progression, transcription, and differentiation. Currently, there are 25 known members of the S100 protein family, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group within the calcium-binding protein family known as EF-hand. Interestingly, 14 of these S100 genes are located on human chromosome 1q21, an area known for genomic instability. Research studies have shown a significant correlation between abnormal S100 protein expression and the progression of cancer. S100 proteins primarily function in immune responses across various tissues, but they also play a role in neuronal development. Each S100 monomer contains two EF-hand motifs and has the capability to bind up to two calcium ions, or sometimes other divalent cations. Structurally, S100 proteins form antiparallel homo- or heterodimers and rely on the presence of calcium, although some interactions can occur in a calcium-independent manner. Despite their similarities, individual S100 members have distinct tissue distribution, cellular localization, and unique protein binding partners, indicating their specific roles in various signaling pathways. Moreover, some S100 proteins function not only intracellularly but also act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities. One prominent member of the S100 protein family is S100B, which is abundantly expressed in astrocytes and serves as a marker for these cells in studies of the central nervous system. S100B is also found in immature and mature myelinating oligodendrocytes that express chondroitin sulfate proteoglycan (NG2).
Other names	： Protein S100-B or S100 calcium-binding protein beta (NEF) is a protein chain known as S-100 protein subunit beta or S100 calcium binding protein beta chain. It is also referred to as S100B or S100beta. This protein, coded by the S100B_HUMAN gene, belongs to the S100 family of calcium-binding proteins. It is predominantly expressed in neural tissues. S100B, also known as protein S100 B, protein S100-B, or S100 calcium binding protein beta chain, belongs to the S100 family of calcium-binding proteins. It is encoded by the S100B_HUMAN gene and is predominantly expressed in neural tissues. This protein is alternatively referred to as S-100 protein subunit beta, S 100 protein beta chain, or S100 calcium-binding protein B. Its functions and interactions are linked to its ability to bind calcium ions. In summary, S100B or protein S100-B is a calcium-binding protein found in neural tissues. It is often referred to as S100 calcium binding protein beta chain, S-100 protein beta chain, or S100 calcium-binding protein B. Encoded by the S100B_HUMAN gene, it is a member of the S100 protein family. Its role and interactions are associated with its calcium-binding ability.
Source	Rabbit
Specificity	Total S100B can be detected using S100B Antibody, which is capable of detecting endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Rabbit;Pig;Dog;Horse;Sheep;Bovine
Antigen	S100B
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human S100B.
MW	11kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Tissue specificity	Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P04271