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Rabbit Anti-NEIL1 Polyclonal Antibody#abs141120

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Description

Catalog-specification	Delivery time	USD price
abs141120-100ug	1-2 Weeks	301.0
abs141120-50ug	1-2 Weeks	201.0

Overview
catalog	abs141120
Other names	Neil1, also known as DNA-(apurinic or apyrimidinic site) lyase Neil1 or DNA glycosylase/AP lyase Neil1, is an important enzyme involved in DNA repair mechanisms. Endonuclease 8-like 1, also referred to as Endonuclease VIII-like 1, is another name for the same enzyme. NEH1, NEI1 and FLJ22402 are alternative names for Neil1. The role of Neil1 is to locate and remove damaged DNA bases, such as those caused by oxidative stress and ionizing radiation, resulting in an apurinic or apyrimidinic site. These sites, also known as abasic sites, are vulnerable to DNA strand breakages and mutations. Neil1 is crucial in maintaining the genomic stability of the cells by repairing and minimizing DNA damage. Nei endonuclease VIII-like 1 (E. coli) is the bacterial homolog of Neil1 and shares a highly homologous sequence. Nei-like protein 1 is another alias for Neil1. The detailed structure and catalytic mechanism of Neil1 have been widely studied, particularly in the domain of base excision repair enzymes. Overall, the Neil1 enzyme is a vital component in maintaining the stability of our DNA and protecting us against various forms of DNA damage. Its importance in DNA repair mechanisms makes it an excellent target for therapeutic strategies against cancer and aging-related diseases.
Source	Rabbit
Specificity	The NEIL1 Antibody is capable of detecting the complete amounts of NEIL1 present naturally within an individual's body.
Species Reactivity	Human
Antigen	NEIL1
Application	The appropriate dilution for Western blot is 1:500 to 1:1000, while for immunofluorescence/immunocytochemistry, it is 1:100 to 1:500. If you are using ELISA with peptide, the suggested dilution is 1:20000 to 1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results in your experiments. By using the appropriate dilution, you can achieve optimal detection of your target protein or molecule.
Immunogen	A synthesized peptide.
MW	44 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. In this process, impurities were removed, resulting in a highly purified antiserum that is now ready for use.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to store for one year at -20 °C. Repeated freeze/thaw cycles should be avoided.
Storage buffer	The storage condition for Rabbit IgG is as follows: it should be stored at -20°C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product has a shelf life of 12 months from the date of receipt. The content provided is rearranged to present the information in a slightly different format while maintaining the original information.
Target
Background	Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
Tissue specificity	Ubiquitous.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	Q96FI4