Phospho-Ataxin-3 (Ser256) Rabbit Polyclonal Antibody#abs140071
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Description
| Catalog-specification | Delivery time | USD price |
abs140071-100ug | 1-2 Weeks | 301.0 |
abs140071-50ug | 1-2 Weeks | 201.0 |
Dear valued customer, please note that the price mentioned in the previous message is for reference purposes only. For accurate and detailed pricing information, we kindly request you to contact our seller Vecent. They will be happy to assist you with any queries you may have. Thank you for your understanding and cooperation.
| Overview | |
catalog | abs140071 |
| Other names | Spinocerebellar ataxia 3 protein, also known as ataxin 3, is encoded by the ATXN3 gene. This gene is associated with various conditions, including Machado Joseph disease (also known as spinocerebellar ataxia 3), olivopontocerebellar ataxia 3, and autosomal dominant ataxin 3. Other variants of ataxin 3, such as ataxin 3 variant h, ataxin 3 variant m, and ataxin 3 variant ref, have been identified. Ataxin 3 is also referred to as Josephin and is the protein product of the ATXN3 gene. It plays a crucial role in the development and progression of Machado Joseph disease, an inherited neurodegenerative disorder. Additionally, it is involved in the pathogenesis of olivopontocerebellar ataxia 3, another form of ataxia. The ATXN3 gene, also known as MJD gene, codes for ataxin 3. Mutations in this gene result in the production of an abnormal form of the protein, leading to the manifestation of Machado Joseph disease. This condition is characterized by progressive impairment of motor control and coordination. In summary, ataxin 3 and the associated ATXN3 gene are central to the development of Machado Joseph disease and other related conditions. Further research is necessary to understand the precise mechanisms by which ataxin 3 functions and its role in disease progression. |
| Source | Rabbit |
| Specificity | The Ataxin-3 (Phospho-Ser256) Antibody is specifically designed to recognize phosphorylated Ataxin-3 at Ser256. It can detect only those endogenous levels of Ataxin-3 that are phosphorylated at this particular residue. The antibody is highly specific and sensitive in its detection of the phosphorylated form of Ataxin-3. This makes it an ideal tool for studying the role of Ataxin-3 in various biological processes where phosphorylation is involved. By using this antibody, researchers can gain insights into the mechanisms underlying the regulation of Ataxin-3 function through phosphorylation at Ser256. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse; |
| Antigen | Ataxin 3 |
| Application | The recommended dilution for western blot is 1:1000-3000, for immunohistochemistry is 1:50-1:200, and for ELISA with peptide is 1:20000-1:40000. Please note that this generated content is based on the original text information, but presented in a different manner. |
| Immunogen | A peptide synthesized from human Ataxin-3 (with Phospho-Ser256) has been developed which bears a strong similarity to the original protein. This peptide has been carefully designed to ensure that it is as effective as possible in addressing the underlying biological mechanisms associated with Ataxin-3. Its development has been based on a deep understanding of the structure and function of the protein, as well as an extensive knowledge of the biomedical field in which it operates. Overall, this synthesized peptide represents an exciting and promising development in the ongoing quest to identify effective treatments for Ataxin-3-related disorders. |
| MW | 43kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The purification process of the antibody involves sequential chromatography on affinity columns containing phospho- and non-phospho-peptides. This process ensures the purification of the antibody from rabbit serum, resulting in a highly purified product. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates (PubMed:12297501, PubMed:17696782, PubMed:23625928, PubMed:28445460, PubMed:16118278). Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins (PubMed:17696782). Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (By similarity). Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription (PubMed:12297501). Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1 (PubMed:28445460). |
| Tissue specificity | Ubiquitous. |
| Posttranslational modification | Monoubiquitinated N-terminally by UBE2W, possibly leading to activate the deubiquitinating enzyme activity (PubMed:23696636). |
| Celluar localization | Cytosol;Mitochondrion;Nucleus;Plasma Membrane; |
| UniPort | P54252 |
Western blot analysis Ataxin-3 (Phospho-Ser256) using LPS treated HeLa whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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