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Rabbit Anti-EIF3D Polyclonal Antibody#abs141133

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Description

Catalog-specification	Delivery time	USD price
abs141133-100ug	1-2 Weeks	301.0
abs141133-50ug	1-2 Weeks	201.0

Overview
catalog	abs141133
Other names	The protein eIF 3 zeta, also known as eIF-3-zeta or eIF3 p66, is a subunit of the eukaryotic translation initiation factor 3 complex. This protein plays an important role in the initiation of protein synthesis by facilitating the binding of the 40S ribosomal subunit to the mRNA molecule. In humans, eIF 3 zeta is encoded by the EIF3D gene and is also known as EIF3S7 or eIF3p66. The eIF 3 zeta protein is a 66/67kDa protein that is composed of 572 amino acids. It contains a WD repeat domain, which is a common protein domain that is often involved in protein-protein interactions. This domain is believed to play a role in the regulation of eIF 3 zeta's activity. In addition to its role in protein synthesis, eIF 3 zeta has also been implicated in a number of other cellular processes. Studies have suggested that it may be involved in DNA damage response, the regulation of cell growth and differentiation, and the modulation of cell signaling pathways. Overall, eIF 3 zeta is an important protein that plays a critical role in the regulation of protein synthesis and a variety of other cellular processes. Further research is needed to fully understand its functions and mechanisms of action.
Source	Rabbit
Specificity	The EIF3D Antibody is capable of detecting total EIF3D levels within the body. This means that it is specifically able to identify the endogenous levels of this substance. By using this antibody, researchers and healthcare professionals can gain valuable insights into the presence of EIF3D within tissue and its potential role in various biological processes. It is important to note that this antibody is highly accurate and precise, making it a valuable tool in the study of EIF3D and its impact on health and disease.
Species Reactivity	Human;Mouse
Antigen	EIF3D
Application	For Western Blotting, the recommended dilution range is 1:500 to 1:1000. For Immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended. When performing Immunofluorescence or Immunocytochemistry, the suggested dilution range is 1:100 to 1:500. For ELISA using the peptide, a range of 1:20000 to 1:40000 dilution is recommended. Please note that these are general guidelines and optimal dilutions may vary for specific experiments.
Immunogen	A synthesized peptide.
MW	64 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. It was a highly effective method for isolating the desired protein and removing any contaminants. The resin's affinity for the targeted peptide allowed for the specific material to be captured and purified to a high degree. This resulted in a purified antiserum that was ready for further analysis or use in downstream applications. Overall, the peptide affinity chromatography using SulfoLink™ Coupling Resin was a successful and efficient method for purifying the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity and effectiveness of this product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to note that repeated freeze/thaw cycles should be avoided in order to maintain the integrity of the product. By following these storage instructions, you can be confident in the quality and performance of this product for its intended use.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline (pH 7.4) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20 °C for optimal preservation. It is important to note that this product is stable for a period of 12 months from the date of receipt.
Target
Background	mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs (PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:18599441, PubMed:25849773). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs (PubMed:27462815).
Celluar localization	Cytosol;
UniPort	O15371