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Rabbit Anti-RORA Polyclonal Antibody#abs133463

Please note that the price provided is solely for your reference. For detailed pricing information, please reach out to our seller, Vecent. Feel free to contact Vecent for further assistance. Western blot analysis of extracts from HeLa cells and COS-7 cells, using RORA antibody. This product is...

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Description

Catalog-specification	Delivery time	USD price
abs133463-100ug	1-2 Weeks	301.0
abs133463-50ug	1-2 Weeks	201.0

Please note that the price provided is solely for your reference. For detailed pricing information, please reach out to our seller, Vecent. Feel free to contact Vecent for further assistance.

Overview
catalog	abs133463
Other names	ROR-alpha, also known as RZR-alpha or Retinoid-related orphan receptor-alpha, is a nuclear receptor belonging to the subfamily 1 group F member 1. It is encoded by the NR1F1 gene and plays a crucial role in regulating gene expression patterns. ROR-alpha is involved in a wide range of biological processes including circadian rhythm regulation, immune system function, and metabolism. The protein is highly conserved across species, with homologs found in mammals, birds, and reptiles. The ROR-alpha protein is a target for drug development, as it has been associated with various diseases such as cancer, diabetes, and autoimmune disorders. Current research is focused on characterizing the molecular mechanisms underlying ROR-alpha function and developing specific modulators to target this protein for therapeutic interventions.
Source	Rabbit
Specificity	The detection of total RORA is possible using the RORA Antibody, which can accurately identify endogenous levels of this protein.
Species Reactivity	Human
Antigen	RORA
Application	The dilution ranges for WB are 1:500~1:1000, for IHC are 1:50~1:200, for IF/ICC are 1:100~1:500, and for ELISA (peptide) are 1:20000~1:40000. It is important to note that these dilutions are based on the original text information provided.
Immunogen	A synthesized peptide.
MW	60 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the purification process of the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is important to store it at a temperature of -20 °C for a year. Ensure that the product is protected from repeated freezing and thawing cycles. It is crucial to follow the recommended storage guidelines to prevent any degradation of the product. Keep the product in a safe storage area where the temperature can be maintained at the recommended level. Make sure to check the temperature regularly to avoid any fluctuation that may harm the product. With proper storage, the product can retain its integrity and efficacy for a longer period of time.
Storage buffer	Store Rabbit IgG in phosphate buffered saline containing 150mM NaCl, pH 7.4, 0.02% sodium azide, and 50% glycerol at -20 °C. This stable storage condition ensures the antibody's integrity for up to 12 months from the date of receipt.
Target
Background	Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of embryonic development, cellular differentiation, immunity, circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target genes regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates genes involved in photoreceptor development including OPN1SW, OPN1SM and ARR3 and skeletal muscle development with MYOD1. Required for proper cerebellum development, regulates SHH gene expression, among others, to induce granule cells proliferation as well as expression of genes involved in calcium-mediated signal transduction. Regulates the circadian expression of several clock genes, including CLOCK, ARNTL/BMAL1, NPAS2 and CRY1. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORA-mediated activation of clock genes expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Regulates genes involved in lipid metabolism such as apolipoproteins APOA1, APOA5, APOC3 and PPARG. In liver, has specific and redundant functions with RORC as positive or negative modulator of expression of genes encoding phase I and phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as CYP7B1 and SULT2A1. Induces a rhythmic expression of some of these genes. In addition, interplays functionally with NR1H2 and NR1H3 for the regulation of genes involved in cholesterol metabolism. Also involved in the regulation of hepatic glucose metabolism through the modulation of G6PC and PCK1. In adipose tissue, plays a role as negative regulator of adipocyte differentiation, probably acting through dual mechanisms. May suppress CEBPB-dependent adipogenesis through direct interaction and PPARG-dependent adipogenesis through competition for DNA-binding. Downstream of IL6 and TGFB and synergistically with RORC isoform 2, is implicated in the lineage specification of uncommitted CD4+ T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. Involved in hypoxia signaling by interacting with and activating the transcriptional activity of HIF1A. May inhibit cell growth in response to cellular stress. May exert an anti-inflammatory role by inducing CHUK expression and inhibiting NF-kappa-B signaling.
Tissue specificity	Widely expressed in a number of tissues. Expressed in both regulatory T-cells (Treg) and effector T-cells (Teff).
Posttranslational modification	Phosphorylation by conventional PKCs in neurons inhibits transcriptional activity. Phosphorylated on Thr-183 by MAPK1/ERK1 in vitro.Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIAS4 but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.Ubiquitinated, leading to its degradation by the proteasome. Proteasomal degradation is required for efficient transcriptional activity and is prevented by HR.Isoform 1: monomethylated at Lys-38 by EZH2, this creates a degron recognized by a DCX (DDB1-DCAF1/VPRBP-CUL4A-RBX1) E3 ubiquitin ligase complex.
Celluar localization	Nucleus;
UniPort	P35398