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Rabbit Anti-DAPK1 Polyclonal Antibody#abs137507

Description

Catalog-specification	Delivery time	USD price
abs137507-100ug	1-2 Weeks	301.0
abs137507-50ug	1-2 Weeks	201.0

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Overview
catalog	abs137507
Other names	DAPK1, also known as DAP kinase 1 or Death Associated Protein Kinase 1, is a protein encoded by the DAPK1 gene in humans. It belongs to the family of death-associated protein kinases and is involved in the regulation of various cellular events such as apoptosis, autophagy, and differentiation. The DAPK1 protein has a molecular weight of approximately 160 kDa and contains several functional domains including a kinase domain, a calmodulin-binding domain, and a death domain. DAPK1 has been implicated in numerous diseases including cancer, Alzheimer's disease, and autoimmune disorders. It is expressed in various tissues such as the brain, heart, and lung and is subject to regulation via multiple post-translational modifications. Overall, DAPK1 is a highly versatile protein with broad implications for human health and disease.
Source	Rabbit
Specificity	The DAPK1 Antibody is capable of recognizing total DAPK1 levels that are naturally present within an organism. This detection occurs at the endogenous level. To create similar content, the information provided in the original text will be reworked without directly copying it.
Species Reactivity	Human;Mouse;Rat
Antigen	DAPK1
Application	The recommended dilutions for Western Blot (WB) range from 1:1000 to 1:3000, while for Immunohistochemistry (IHC) it is suggested to use a dilution of 1:200. For ELISA using a peptide, the recommended dilution range is from 1:20000 to 1:40000. It is important to follow these dilutions to ensure accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human DAPK1.
MW	160kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated cycles of freezing and thawing. By following these guidelines, you can ensure that the product remains in optimal condition for an extended period of time.
Storage buffer	Store Rabbit IgG in phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol at -20 °C. It remains stable for 12 months from the date of receipt.
Target
Background	Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
Tissue specificity	Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
Posttranslational modification	Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.Removal of the C-terminal tail of isoform 2 (corresponding to amino acids 296-337 of isoform 2) by proteolytic cleavage stimulates maximally its membrane-blebbing function.In response to mitogenic stimulation (PMA or EGF), phosphorylated at Ser-289; phosphorylation suppresses DAPK1 pro-apoptotic function. Autophosphorylation at Ser-308 inhibits its catalytic activity. Phosphorylation at Ser-734 by MAPK1 increases its catalytic activity and promotes cytoplasmic retention of MAPK1. Endoplasmic-stress can cause dephosphorylation at Ser-308.
Celluar localization	Cytoskeleton;Plasma Membrane;
UniPort	P53355