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Rabbit Anti-CD46 Polyclonal Antibody#abs136051

Please note that the price mentioned above is for your reference only. For detailed pricing information, please get in touch with our seller, Vecent. Feel free to contact Vecent for any queries regarding the prices. Western blot analysis of extracts of various celllines, using CD46 antibody....

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Description

Catalog-specification	Delivery time	USD price
abs136051-100ug	1-2 Weeks	301.0
abs136051-50ug	1-2 Weeks	201.0

Overview
catalog	abs136051
Description	CD46, a regulatory component of the complement system, stands as a pivotal type I membrane protein. Acting as a shield, it exhibits cofactor activity, which joins forces with serum factor I to deactivate complement components C3b and C4b, safeguarding host cells from imminent damage. Moreover, CD46 unveils its versatility by assuming the role of a receptor for various infectious agents such as the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Not only does it act as a gatekeeper for invading pathogens, but CD46 also demonstrates its involvement in the conjugation of spermatozoa with the oocyte during the intricate process of fertilization. Encoded within the genome's magnetic tape on chromosome 1q32, CD46 resides in a cluster alongside other proteins that serve as the solid foundation of the complement system.
Other names	The CD46 antigen, also referred to as the complement membrane cofactor protein, is a molecule identified by monoclonal antibody TRA 2 10. This protein, encoded by the MCP gene in humans, functions as a regulator of the complement system. It is also recognized as the measles virus receptor and trophoblast-lymphocyte cross-reactive antigen. In addition to its role in complement regulation, CD46 has been implicated in a variety of biological functions, including immune cell activation, pathogen recognition, and tumor suppression. The protein is widely expressed on the surface of many cell types, including leukocytes, epithelial cells, and trophoblasts. Interestingly, CD46 has a dual function in immune regulation. On one hand, it can serve as a co-stimulatory molecule to promote immune responses. On the other hand, CD46 can also act as a negative regulator of immune activation by dampening T cell proliferation and cytokine production. The importance of CD46 in human health and disease is underscored by its association with a variety of conditions, including autoimmune disorders, infectious diseases, and cancer. Further research into the biology of CD46 and its downstream signaling pathways may reveal new insights into the pathogenesis of these conditions and offer new therapeutic targets for treatment.
Source	Rabbit
Specificity	The CD46 Antibody is effective in identifying the complete levels of CD46 in its natural state within cells.
Species Reactivity	Human;Mouse;Rat
Antigen	CD46
Application	For Western blotting, the recommended dilution range is 1:500-1:2000. When using immunohistochemistry, a dilution range of 1:50-1:200 is suggested. For ELISA using peptides, a dilution range of 1:20000-1:40000 is recommended. It's important to follow these recommended dilutions to obtain reliable and accurate results.
Immunogen	A synthesized peptide derived from human CD46.
MW	43kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
Tissue specificity	Expressed by all cells except erythrocytes.
Posttranslational modification	N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.
Celluar localization	Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P15529