Phospho-p130 Cas (Tyr410) Rabbit Polyclonal Antibody#abs130975

abs130975-50ug

1-2 Weeks

201

abs130975-100ug

1-2 Weeks

301

Description

This protein acts as a hub for multiple protein-protein interactions and is heavily involved in cell signaling processes revolving around tyrosine kinases and cell adhesion. Its essential role in coordinating these events makes it a pivotal factor in inducing cell migration.

Other names

p130Cas is a protein that plays a significant role in breast cancer anti-estrogen resistance. It belongs to the CAS scaffolding protein family and is also known as BCAR1 or BCAR1_HUMAN. The primary function of p130Cas is to regulate cellular activities associated with breast cancer progression and resistance to estrogen therapy.
This protein, encoded by the BCAR1 gene, is involved in signal transduction pathways and acts as a link between extracellular signals and intracellular responses. It is closely associated with the Crk protein and forms a complex known as Crk-Cas. This complex is responsible for transmitting signals from the cell surface to the nucleus, leading to various cellular processes and ultimately affecting cancer cell behavior.
p130Cas is a multifunctional protein that acts as a substrate for several kinases, including Src and focal adhesion kinase (FAK). Phosphorylation of p130Cas at specific sites results in the recruitment of other signaling molecules and the activation of downstream signaling pathways. These signaling events promote cell survival, migration, and invasion, which are characteristic features of cancer progression and resistance to anti-estrogen therapies.
Furthermore, p130Cas has been identified as a key player in mediating cell adhesion and cytoskeletal rearrangement, both of which are essential for cancer cell motility and metastasis. It interacts with various proteins, such as actin, vinculin, and paxillin, to regulate cell shape, movement, and adhesion to the extracellular matrix.
In summary, p130Cas is a critical player in breast cancer anti-estrogen resistance. Its involvement in multiple signaling pathways and cellular processes makes it an attractive target for developing novel therapeutic strategies against breast cancer. Further research on the functional mechanisms of p130Cas will undoubtedly contribute to a better understanding of breast cancer biology and the development of more effective treatments in the future.

Source

Rabbit

Specificity

The Antibody for Phospho-p130 Cas (Tyr410) is able to identify p130 Cas at its natural levels only when it has been phosphorylated at Tyrosine 410. This means it is able to detect the specific modification of the p130 Cas that occurs at this site.

Predictive reaction species

Pig

Application

For detecting our target protein, recommended dilutions for western blotting are 1:500 to 1:2000, for immunohistochemistry are 1:50 to 1:200, for immunofluorescence and immunocytochemistry are 1:100 to 1:500, and for ELISA using peptide antigen are 1:20000 to 1:40000. Please ensure to follow the recommended protocol and optimize the dilution according to your experimental conditions.

A peptide has been synthesized from human p130 Cas, specifically focusing on the phosphorylation site of Tyrosine 410. The synthesized peptide closely resembles the original sequence and has been created using advanced laboratory techniques. This peptide is expected to serve as a crucial tool for researchers in further understanding the role of p130 Cas phosphorylation at Tyrosine 410 and its implications in various cellular processes. Overall, the synthesized peptide is a promising contribution to the scientific community's ongoing efforts towards advancing our understanding of human biology.

Properties

1mg/ml

Purification

We performed sequential chromatography on phospho- and non-phospho-peptide affinity columns to purify the antibody from rabbit serum. The purification process involved affinity purification using purified rabbit serum.

Clonality

Polyclonal Antibody

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to multiple cycles of freezing and thawing.

Storage buffer

This rabbit IgG is stored in a buffer containing phosphate and saline, with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, and can be stored safely at -20°C. It can remain stable for up to 12 months from the day of its receipt.

Target

Background

This protein acts as a crucial mediator of signal transduction in tyrosine kinase-related cellular signaling pathways, particularly those involved in cell adhesion. It is also associated with the promotion of cell migration. Breast cancer cells that exhibit high levels of this protein have demonstrated resistance to antiestrogen treatment.

Tissue specificity

Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

Celluar localization

Cytoskeleton;Cytosol;

P56945