Phospho-PTEN (Ser370) Rabbit Polyclonal Antibody#abs130976

abs130976-50ug

1-2 Weeks

201

abs130976-100ug

1-2 Weeks

301

Description

PTEN, a tumor suppressor, is a critical enzyme known as a phosphoinositide 3-phosphatase (PIP3). It plays a significant role in various human cancers by dephosphorylating inositol phospholipids which are produced as a result of the activation of the phosphoinositide 3 kinase (PI3K). Its function as a phosphatase allows it to regulate the signaling pathway involving PI3K and PIP3, ultimately impacting cellular processes and contributing to the development of tumors. Considering its wide involvement in different types of cancer, PTEN serves as an important target for therapeutic interventions aimed at restoring proper regulation of the PI3K signaling pathway.

Other names

PTEN, also known as phosphatase and tensin homolog, is a crucial protein involved in various biological processes. It is encoded by the PTEN gene located on chromosome 10. PTEN functions as a dual-specificity protein phosphatase and a phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase. Its role in regulating the PI3K/AKT/mTOR signaling pathway makes it a significant tumor suppressor.
Mutations in the PTEN gene have been observed in multiple advanced cancers, highlighting its importance in maintaining cellular homeostasis and preventing tumorigenesis. In fact, studies have shown that the loss or inactivation of PTEN can lead to uncontrolled cell growth and division, contributing to the development and progression of various malignancies.
Due to its critical role in cellular signaling, PTEN has been the subject of extensive research. Its structural features and functional domains, such as the phosphatase domain and the C2 domain, have been extensively studied to understand the molecular mechanisms underlying its tumor-suppressive functions.
Furthermore, PTEN is not the only member of its family. There are other related proteins like PTEN-like protein (PTEN2) and tensin homolog deleted on chromosome 10-like protein (TEN2). These proteins possess similar domains and functions, suggesting a conserved role in cellular processes.
In conclusion, PTEN is an important protein involved in regulating cellular signaling pathways and maintaining genomic stability. Its dysfunction or loss is associated with the development of various advanced cancers. Further research on PTEN and its related proteins may provide valuable insights into the development of targeted therapies for cancer treatment.

Source

Rabbit

Specificity

The detection of endogenous levels of PTEN using the Phospho-PTEN (Ser370) Antibody is specifically limited to instances where PTEN is phosphorylated at Serine 370. To reiterate, this antibody is designed to target and recognize PTEN only when it is in its phosphorylated state at Serine 370.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Horse;Bovine

Application

The recommended antibody dilution for Western Blot is between 1:500-1:2000, for Immunohistochemistry it is between 1:50-1:200, for Immunofluorescence and Immunocytochemistry it is between 1:100-1:500, and for ELISA assays using peptide antigens, a dilution of 1:20000-1:40000 is recommended. These optimized dilutions will ensure the best results for your experiments, providing reliable and specific detection of your target protein. It is important to note that the actual optimal dilution may vary depending on the sample type, antigen density, and experimental conditions, so it is recommended to perform a dilution series to determine the best dilution for your specific application.

PTEN, a significant protein in humans, can be utilized to synthesize a peptide encompassing the region surrounding Serine 370, the phosphorylation site. To maintain the derived content's similarity to the original, it is crucial to rearrange the provided information and ensure that the generated content remains rooted in the original text. Please note that the aim is not to converse as per the ChapGPT content generation approach, but rather to speak in a manner that produces text distinct from the language model.

Properties

1mg/ml

Purification

The purified rabbit serum was utilized to obtain the antibody through a sequential chromatography process on affinity columns specific to phospho- and non-phospho-peptides. The resulting content is highly similar to the original text but with a rearrangement of the information.

Clonality

Polyclonal Antibody

To maintain quality, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4. It also includes 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The stability of the product is guaranteed for a period of 12 months from the date of receipt.

Target

Background

This protein is a tumor suppressor that functions as a dual-specificity protein phosphatase. It is responsible for removing phosphate groups from tyrosine-, serine-, and threonine-phosphorylated proteins. Additionally, it acts as a lipid phosphatase, removing the phosphate from the inositol ring in various phosphatidylinositol substrates, including PtdIns(3,4,5)P3, PtdIns(3,4)P2, PtdIns3P, and Ins(1,3,4,5)P4. The lipid phosphatase function is essential for its tumor suppressor activity.
This protein works to inhibit the PI3K-AKT/PKB signaling pathway, thereby regulating cell cycle progression and cell survival. It cooperates with AIP1 to suppress AKT1 activation. Furthermore, it dephosphorylates tyrosine-phosphorylated focal adhesion kinase, which prevents cell migration, integrin-mediated cell spreading, and focal adhesion formation.
The protein is also critical for the AKT-mTOR signaling pathway in adult neurogenesis. Specifically, it controls the tempo of newborn neurons' integration, including neuron positioning, dendritic development, and synapse formation. It may negatively regulate insulin signaling and glucose metabolism in adipose tissue.
The nuclear monoubiquitinated form of the protein exhibits greater apoptotic potential, while the cytoplasmic nonubiquitinated form has less tumor suppressive ability. In motile cells, it prevents the formation of lateral pseudopods, which promotes cell polarization and directed movement.

Tissue specificity

Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Celluar localization

Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;

P60484