Phospho-HDAC4 (Ser632) Rabbit Polyclonal Antibody#abs130974

abs130974-50ug

1-2 Weeks

201

abs130974-100ug

1-2 Weeks

301

Description

HDAC4 belongs to subfamily 2 of the histone deacetylase family and acts as a transcriptional regulator. It exerts its regulatory function by deacetylating lysine residues located on the N-terminal region of core histones H2A, H2B, H3, and H4.

Other names

Here are some different ways to rearrange the information:
1. Histone deacetylase 4, also known as HDAC4 or HDAC A, is an enzyme encoded by the KIAA0288 gene. Its EC number is 3.5.1.98 and it is involved in the deacetylation of histone proteins. Another name for HDAC4 is HA6116, and it is sometimes abbreviated as HD4 or HDACA.
2. The human protein HDAC4, also called histone deacetylase A, is encoded by the KIAA0288 gene and has the EC number 3.5.1.98. It plays a role in the deacetylation of histone proteins and is sometimes referred to as HD4, HDACA, or HA6116.
3. HDAC4 is an enzyme that belongs to the histone deacetylase family and is encoded by the KIAA0288 gene in humans. It has the EC number 3.5.1.98 and is responsible for removing acetyl groups from histones. Other names for HDAC4 include HD4, HDACA, HA6116, and histone deacetylase A.
4. The gene KIAA0288 encodes a protein in humans called HDAC4, which is also known as histone deacetylase A. This enzyme has the EC number 3.5.1.98 and is involved in the deacetylation of histones. HDAC4 is sometimes abbreviated as HD4, HDACA, or HA6116.
5. Histone deacetylase 4, or HDAC4, is a human enzyme that is encoded by the KIAA0288 gene. Its EC number is 3.5.1.98 and it plays a role in the deacetylation of histone proteins. HDAC4 is also referred to as HDACA, HD4, HA6116, or histone deacetylase A.

Source

Rabbit

Specificity

The Phospho-HDAC4 (Ser632) Antibody is specifically designed to identify HDAC4 levels that are phosphorylated at Serine 632. Only endogenous levels of HDAC4 are detected by this antibody when it is phosphorylated at this specific site. Rearranging the original content, this information can be expressed in a slightly different manner.

Predictive reaction species

Xenopus;Zebrafish;Chicken;Pig;Dog;Sheep;Bovine

Application

Our recommended dilution ranges for Western Blot (WB) are 1:500-1:2000, for Immunofluorescence (IF) and Immunocytochemistry (ICC) are 1:100-1:500, and for ELISA using peptide are 1:20000-1:40000. Please note that the generated content will be a highly similar version of the original information but presented in a different format.

A peptide that has been created through synthesis, using human HDAC4 as the blueprint and focusing on the phosphorylation site of Serine 632. To ensure accuracy, the resulting peptide closely mimics the original sequence from which it was derived.

Properties

1mg/ml

Purification

Sequential chromatography on phospho- and non-phospho-peptide affinity columns was used to purify the antibody from purified rabbit serum, resulting in a highly purified product.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can compromise its efficacy.

Storage buffer

The Rabbit IgG is suspended in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain its stability, it should be stored at -20 °C. The product remains viable for a period of 12 months from the date of receipt.

Target

Background

Histone deacetylases are responsible for removing acetyl groups from lysine residues on core histones' N-terminal regions, including H2A, H2B, H3, and H4. This process helps to establish epigenetic repression, playing a vital role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases function through the formation of large multiprotein complexes. They are involved in muscle maturation through interaction with myocyte enhancer factors like MEF2A, MEF2C, and MEF2D. Moreover, these enzymes aid in regulating ESR1 expression in breast cancer through MTA1-mediated epigenetic mechanisms. By deacetylating HSPA1A and HSPA1B at Lys-77, histone deacetylases cause these proteins to preferentially bind to co-chaperone STUB1. Overall, histone deacetylases play essential roles in various cellular processes and have significant implications for health and disease.

Tissue specificity

Ubiquitous.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

P56524