Rabbit Anti-CD133 Polyclonal Antibody#abs131197

abs131197-50ug

1-2 Weeks

201

abs131197-100ug

1-2 Weeks

301

Description

CD133, initially identified as AC133, is a type of glycoprotein that is also known as Prominin 1 (PROM1) in both humans and rodents. Its specific function is still unclear. CD133 belongs to the group of pentaspan transmembrane glycoproteins, which are characterized by having five transmembrane domains and are localized to cellular protrusions. This glycoprotein is predominantly expressed in hematopoietic stem cells.

Other names

Antigen AC133, also known as CD133 or hematopoietic stem cell antigen, is a protein encoded by the AC133 gene. It is also referred to as CORD12, hProminin, MCDR2, MSTP061, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, PROM1, PROM1_HUMAN, Prominin I, Prominin like 1, Prominin like protein 1 precursor, Prominin mouse like 1, Prominin-1, Prominin-like protein 1, Prominin1, PROML1, RP41, and STGD4. This antigen is an important marker for stem cells and plays a significant role in various biological processes. By rearranging the above information, we can create a similar content with a different structure.

Source

Rabbit

Specificity

The total amount of CD133 present in a biological sample can be accurately determined using the CD133 Antibody, which specifically recognizes and detects the endogenous levels of CD133.

Application

For Western blotting, the recommended dilution range for this antibody is between 1:500 and 1:2000. In the case of immunohistochemistry, a dilution range of 1:50 to 1:200 is suggested. When using this antibody for immunofluorescence, a 1:200 dilution is optimal. For ELISA using a peptide substrate, the recommended dilution is between 1:20000 and 1:40000. These recommended dilution ranges have been determined through testing and are intended to provide the best results when using this antibody in various applications.

A synthesized peptide derived from human CD133.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized in peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. However, it is important to avoid subjecting the product to repeated cycles of freezing and thawing as this can have a detrimental effect on its quality. Therefore, it is advisable to store the product in a freezer that remains consistently at the recommended temperature to maintain its integrity.

Storage buffer

The suggested rearranged content:
To store Rabbit IgG, use phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be kept at a temperature of -20 °C. This product remains stable for a period of 12 months starting from the date of receipt.

Target

Background

This protein has various functions in cell processes such as differentiation, proliferation, and apoptosis according to a study published in PubMed (PubMed:24556617). It has the ability to bind cholesterol in specific regions of the plasma membrane that contain cholesterol. This function suggests that it may be involved in organizing the structure of the apical plasma membrane in epithelial cells. Moreover, during the early development of the retina, it plays a crucial role in regulating the formation of disk-like structures. Additionally, it is implicated in the regulation of signaling pathways such as MAPK and Akt. Interestingly, in neuroblastoma cells, it inhibits cell differentiation, specifically preventing the outgrowth of neurites, in a manner dependent on the RET protein (PubMed:20818439).

Tissue specificity

Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane;

O43490