Rabbit Anti-Smad5 Polyclonal Antibody#abs131196

abs131196-50ug

1-2 Weeks

201

abs131196-100ug

1-2 Weeks

301

Description

SMAD5 is a type of transcriptional modulator that becomes activated when stimulated by BMP (bone morphogenetic proteins) type 1 receptor kinase. This protein acts as a receptor-regulated SMAD (R-SMAD), meaning that it is regulated by the receptor it binds to.

Other names

SMAD5, also known as DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, and Smad5, is a protein that plays a role in various cellular processes. SMAD5_HUMAN is the human gene encoding this protein.
SMAD5 is a key player in the transforming growth factor-beta (TGF-beta) signaling pathway. It belongs to the SMAD family of proteins, which are intracellular mediators of TGF-beta superfamily ligands. SMAD proteins are essential for transmitting signals from the cell surface receptors to the nucleus, where they regulate gene expression.
The function of SMAD5 is highly conserved among species, and it has been found to be involved in embryogenesis, cell proliferation, differentiation, and apoptosis. SMAD5 interacts with various transcription factors and coactivators to regulate the expression of target genes in response to TGF-beta signaling.
Research has shown that SMAD5 plays a critical role in skeletal development and patterning. It is required for the formation and maintenance of the axial skeleton, limb buds, and craniofacial structures. Mutations in the SMAD5 gene have been associated with developmental disorders and skeletal malformations.
Furthermore, SMAD5 has been implicated in cancer progression. It functions as a tumor suppressor in some contexts, inhibiting cell growth and inducing apoptosis. However, in certain types of cancer, SMAD5 can promote tumor growth and metastasis by stimulating angiogenesis and invasiveness.
In summary, SMAD5 is an important protein involved in TGF-beta signaling and a wide range of cellular processes. Its roles in development, disease, and cancer make it an intriguing target for further research and therapeutic interventions.

Source

Rabbit

Specificity

The Smad5 Antibody is capable of identifying total Smad5 in the body at its normal level. The antibody reacts specifically to endogenous levels of Smad5 without cross-reacting with other proteins.

Predictive reaction species

Sheep;Bovine;Dog;Horse;

Application

The dilution ratios for Western blotting range from 1:500 to 1:2000, for immunohistochemistry from 1:50 to 1:200, for immunofluorescence from 1:100 to 1:500, and for ELISA (peptide) from 1:20000 to 1:40000. It is important to note that the provided dilution ratios are based on the original text information.

A synthesized peptide derived from human Smad5.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography. Feel free to ask if you have any further questions.

Clonality

Polyclonal Antibody

Make sure to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid frequent freezing and thawing cycles.

Storage buffer

This solution contains Rabbit IgG in a buffer of phosphate buffered saline at pH 7.4, with 150mM of NaCl, 0.02% sodium azide and 50% glycerol to prevent freezing damage. It should be stored at -20 °C for optimal stability, and can be used for up to 12 months from the receipt date.

Target

Background

SMAD5 is a transcriptional modulator that is activated by BMP, which stands for bone morphogenetic proteins. This protein is considered a receptor-regulated SMAD, or R-SMAD, specifically activated by the type 1 receptor kinase.

Tissue specificity

Ubiquitous.

Celluar localization

Cytosol;Nucleus;

Q99717