Rabbit Anti-PI3 Kinase P85alpha Polyclonal Antibody#abs131198

abs131198-50ug

1-2 Weeks

201

abs131198-100ug

1-2 Weeks

301

Description

The SH2 domain of this protein binds to phosphorylated protein-Tyr kinases, enabling it to act as an adapter. Its primary function is to facilitate the association between the p110 catalytic unit and the plasma membrane. This interaction is crucial for the insulin-induced enhancements in glucose uptake and glycogen synthesis in insulin-sensitive tissues. In addition, this protein is involved in signaling pathways elicited by FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA, PDGFRB, and ITGB2. Its role in these signaling events is vital for the proper transmission of cellular responses to the activation of these receptors.

Other names

Today, we will discuss GRB1, also known as p85 alpha or PI3K regulatory subunit alpha. This protein is a regulatory subunit of the phosphatidylinositol 3-kinase (PI3K) enzyme, which plays a crucial role in various cellular processes, including cell growth, survival, and metabolism. The gene encoding GRB1 is located on chromosome 5 and is expressed in a variety of tissues.
GRB1 consists of several domains, including an SH3 domain, two SH2 domains, and an N-terminal region that binds to the p110 catalytic subunit of PI3K. It serves as a critical linker between receptor tyrosine kinases and PI3K activation, which generates second messenger molecules that stimulate downstream signaling pathways.
Mutations in the GRB1 gene have been associated with various diseases, including cancer, insulin resistance, and autoimmune disorders. In particular, altered activity of PI3K due to mutations in GRB1 has been linked to the development of several types of cancer, including breast, lung, and prostate cancer.
In summary, GRB1 is an essential component of the PI3K enzyme and plays a crucial role in cellular signaling pathways. Understanding its function and regulation may lead to the development of novel therapeutic interventions for a wide range of diseases.

Source

Rabbit

Specificity

The PI3 kinase P85α Antibody is capable of identifying the complete levels of endogenous PI3 kinase P85α.

Predictive reaction species

Rabbit;Chicken;Sheep;Bovine;Horse;Zebrafish;

Application

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For Western blotting (WB), the recommended dilution range is 1:500 to 1:2000. When performing immunohistochemistry (IHC), the appropriate dilution range is 1:50 to 1:200. If you're using ELISA with a peptide, a dilution of 1:20000 to 1:40000 should be used.

Human PI3 kinase P85α can be used to synthesize a peptide with high similarity. Please rearrange the content accordingly to ensure that the generated content is based on the original text information.

Properties

1mg/ml

Purification

Peptide affinity chromatography with SulfoLink™ Coupling Resin was employed to purify the antiserum. Let me provide you with a revised content that maintains the essence of the original information.

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Tissue specificity

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Celluar localization

Cytosol;Endoplasmic reticulum;Nucleus;Plasma Membrane;

P27986