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Rabbit Anti-CCNB1 Polyclonal Antibody#abs136306

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Description

Catalog-specification	Delivery time	USD price
abs136306-50ug	1-2 Weeks	201.0
abs136306-100ug	1-2 Weeks	301.0

Overview
catalog	abs136306
Description	Cyclins, a group of proteins, play a crucial role in activating specific cyclin-dependent kinases (CDKs) needed for the progression of the cell cycle. The transition of all eukaryotic cells into mitosis heavily relies on the activation of cdc2/cdk1 during the G2/M phase. This complex process commences with the binding of cyclin B1, the regulatory subunit, with cdc2/cdk1, leading to the formation of the mitosis-promoting factor (MPF). MPF remains inactive until cdc2/cdk1 is phosphorylated at Thr161 by cdk activating kinase (CAK) and dephosphorylated at Thr14/Tyr15 by cdc25C. The presence of phosphorylation sites (Ser126, 128, 133, and 147) in cyclin B1's cytoplasmic retention signal (CRS) domain helps regulate its movement to the nucleus, enabling nuclear accumulation and initiation of mitosis at the G2/M checkpoint. These sites are believed to be phosphorylated by either MPF or polo-like kinase 1 (PLK1), with Ser133 being the preferred site for PLK1 phosphorylation, and possibly Ser147. When mitosis concludes, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), facilitating cell cycle progression. Studies have demonstrated elevated expression of cyclin B1 in breast, prostate, and non-small cell lung cancers.
Other names	Cyclin B1, also known as CCNB1 or CCNB, is a protein that plays a crucial role in cell division. Specifically, it is involved in controlling the transition from the G2 phase of the cell cycle to the mitotic (M) phase. This means that Cyclin B1 is highly expressed during the M phase, and is needed for the proper progression of mitosis. In humans, the CCNB1 gene encodes the Cyclin B1 protein. Mutations or alterations in this gene have been associated with various human diseases, including cancer. As a result, Cyclin B1 has become a target of research in the development of anti-cancer therapies. Overall, Cyclin B1 is a vital component of the cell cycle machinery, and its function is crucial for maintaining proper cellular division.
Source	Rabbit
Specificity	The CCNB1 Antibody is designed to identify total CCNB1 levels present in the body. This antibody can effectively detect endogenous amounts of CCNB1 protein. For accurate analysis of CCNB1 expression in biological samples, this antibody can be a useful tool.
Species Reactivity	Human;Mouse;Rat
Antigen	CCNB1
Application	The recommended dilutions for WB are 1:500 to 1:2000, for IHC are 1:50 to 1:200, for IF/ICC are 1:100 to 1:500, and for ELISA (peptide) are 1:20000 to 1:40000. To ensure accurate results, it is important to use appropriate concentrations of primary antibody and to optimize the dilution for each individual experiment. Dilution factors may need to be adjusted based on the type of sample and detection method used. With proper standardization, these dilution ranges can serve as a helpful starting point for immunohistochemistry and other antibody-based assays.
Immunogen	A synthetic peptideof human CCNB1.
MW	48kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Tissue specificity	Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Posttranslational modification	Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Celluar localization	Cytoskeleton;Cytosol;Mitochondrion;Nucleus;
UniPort	P14635