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Rabbit Anti-EPHA2 Polyclonal Antibody#abs131333

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Description

Catalog-specification	Delivery time	USD price
abs131333-50ug	1-2 Weeks	201.0
abs131333-100ug	1-2 Weeks	301.0

Overview
catalog	abs131333
Description	EPHA2 is a vital receptor exclusively designed to bind with members of the ephrin-A family, particularly ephrin-A1, -A3, -A4, and -A5. Its significant role in angiogenesis and the formation of new blood vessels in tumors cannot be understated. Phosphorylated EPHA2 plays a crucial role in activating the RAC1 GTPase, promoting vascular endothelial cell migration and assembly in response to EFNA1. This process relies on the recruitment of VAV2, VAV3, and the PI3-kinase p85 subunit. Interestingly, the induction of apoptosis by EPHA2 operates independently of the tumor suppressor protein p53/TP53. Instead, it follows a caspase-8-dependent pathway.
Other names	CTPA; AW545284; ARCC2; CTPP1; CTRCT6; EC 2.7.10.1; Eck; Eph receptor A2; EPHA2; EPHA2_HUMAN; Ephrin receptor; Ephrin receptor EphA2; Ephrin type A receptor 2; Ephrin type-A receptor 2; Epithelial cell receptor protein tyrosine kinase; Epithelial cell kinase; Myk 2; Myk2; Sek 2; Sek2; Soluble EPHA2 variant 1; Tyrosine protein kinase receptor ECK; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2; The listed proteins and Enzyme Commission (EC) number 2.7.10.1 are related to Eph receptor A2, also known as EphA2 or EPHA2. EphA2 is an ephrin receptor and belongs to the Eph family of receptor tyrosine kinases. It is involved in cell signaling, particularly in processes related to cell adhesion, migration, and survival. EphA2 is also known by various other names such as epithelial cell receptor protein tyrosine kinase, Myk 2, Sek 2, and Tyrosine-protein kinase receptor ECK/MPK-5/SEK-2. Additionally, it has a soluble variant known as soluble EPHA2 variant 1. EphA2 plays a crucial role in the development and function of epithelial cells. It regulates cellular processes such as cell-cell communication and tissue organization. Dysregulation of EphA2 has been observed in various diseases, including cancer, where it is implicated in tumor growth, invasion, and metastasis. Understanding the functions and mechanisms of EphA2 and its associated proteins, such as CTPA, AW545284, ARCC2, CTPP1, and CTRCT6, can provide insights into the underlying molecular processes and potential therapeutic targets for various diseases.
Source	Rabbit
Specificity	The EPHA2 Antibody is capable of identifying native levels of total EPHA2.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Bovine;Dog;Horse;
Antigen	EPHA2
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human EPHA2.
MW	108 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
Tissue specificity	Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.
Posttranslational modification	Autophosphorylates. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-930 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-897 by PKB; serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Phosphorylated at Ser-897 in response to TNF by RPS6KA1 and RPS6KA3; RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630). Phosphorylated at Ser-897 by PKA; blocks cell retraction induced by EPHA2 kinase activity (PubMed:27385333). Dephosphorylated by ACP1.Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation (By similarity).
Celluar localization	Plasma Membrane;
UniPort	P29317