Phospho-ATRIP (Ser68) Rabbit Polyclonal Antibody#abs131093

abs131005-50ug

1-2 Weeks

201

abs131005-100ug

1-2 Weeks

301

Description

Checkpoint signaling after DNA damage relies on the presence of ATRIP. This protein is crucial for ATR expression and it may play a role in enhancing its stability. To ensure proper checkpoint activation, ATRIP is a necessary factor that supports the signaling cascade triggered by DNA damage.

Other names

A protein known as AGS1, also referred to as ATIP, is closely linked with ATM and Rad3-related pathways. This protein is referred to as ATM and Rad3-related-interacting protein or ATR-interacting protein. Atrip or ATRIP_HUMAN is another name for it, and it is also recognized by the aliases DKFZp762J2115, FLJ12343, MGC20625, MGC21482, and MGC26740. The function of AGS1 is to interact with ATM and ATR and play a vital role in DNA replication and repair. It is essential for maintaining genomic stability and preventing DNA damage. Scientists are interested in discovering more about this protein and its potential therapeutic applications.

Source

Rabbit

Specificity

The Phospho-ATRIP (Ser68) Antibody recognizes the phosphorylated form of ATRIP at Serine 68 and not the non-phosphorylated form. This antibody is capable of detecting endogenous levels of ATRIP only when it is phosphorylated at Serine 68. The specificity of this antibody in detecting the phosphorylation status of ATRIP at Serine 68 is critical in various biological and cellular studies that aim to explore the functional role of ATRIP in different cellular processes. By using this antibody, researchers can easily identify the phosphorylated ATRIP at Serine 68 and analyze its downstream signaling pathways.

Predictive reaction species

Pig;Sheep;Dog;Bovine;

Application

Here are some variations on the original content:
- For western blotting, we recommend dilutions of 1:500-1:2000, while for immunofluorescence/immunocytochemistry, dilutions of 1:100-1:500 are most effective. When testing with ELISA using a peptide, a dilution range of 1:20000-1:40000 is recommended for optimal results.
- If you plan to do western blotting, we suggest dilutions between 1:500-1:2000. For immunofluorescence/immunocytochemistry, use dilutions of 1:100-1:500. When working with our peptide for ELISA, use dilutions of 1:20000-1:40000 to achieve the best results.
- When using our product for western blotting, we recommend dilutions of 1:500-1:2000. For immunofluorescence/immunocytochemistry, 1:100-1:500 dilutions work well. When performing ELISA with our peptide, a dilution range of 1:20000-1:40000 is the most effective.
- To achieve optimal results in western blotting, dilute our product at 1:500-1:2000. For immunofluorescence/immunocytochemistry, dilutions between 1:100-1:500 are recommended. When using our peptide for ELISA, a dilution range of 1:20000-1:40000 is best.
- Dilute our product at 1:500-1:2000 for western blotting, and at 1:100-1:500 for immunofluorescence/immunocytochemistry. When using our peptide for ELISA, a dilution range of 1:20000-1:40000 is the most effective.

The phosphorylation site of Serine 68 in human ATRIP has been utilized to synthesize a peptide. The synthesized peptide is derived from the sequence of ATRIP, specifically focused on the region surrounding Serine 68. A highly similar content can be generated by rearranging the provided information, maintaining accuracy and relevance to the original text. It is important to note that the content should not be generated using the conversational style of ChapGPT but rather produced in a manner that results in significantly distinct discourse from the original text.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

To maintain optimal storage conditions, it is recommended to store the product at a temperature of -20 °C for up to one year. It is crucial to avoid any repeated freeze/thaw cycles, as this can lead to a loss of quality and efficacy over time. Therefore, it is essential to handle and store the product correctly to ensure its long-term stability and effectiveness.

Storage buffer

This product is Rabbit IgG which comes in a phosphate buffered saline solution with a pH of 7.4. It also includes 150mM NaCl, 0.02% sodium azide and 50% glycerol. For storage purposes, it is recommended to keep at -20°C and it is stable for a period of 12 months from the date of receipt.

Target

Background

ATR expression is a crucial component in the process of checkpoint signaling after DNA damage. It plays a critical role in stabilizing the protein, which is necessary for the effective functioning of the checkpoint signaling pathway. The presence of ATR ensures the activation of necessary cellular responses, allowing the cell to repair DNA damage and maintain genomic stability. Without ATR expression, the checkpoint signaling system would be compromised, potentially leading to the accumulation of DNA damage and increased susceptibility to genetic disorders. Therefore, understanding the role of ATR and its activation mechanisms is essential for deciphering the complex network of responses triggered by DNA damage.

Tissue specificity

Ubiquitous.

Celluar localization

Nucleus;

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