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Rabbit Anti-AR Polyclonal Antibody#abs136303

Please note that the price mentioned above is only a reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of extracts of variouscell lines, using AR antibody. This product is for research use only, not for use in diagnostic prodecures or...

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Description

Catalog-specification	Delivery time	USD price
abs136303-50ug	1-2 Weeks	201.0
abs136303-100ug	1-2 Weeks	301.0

Please note that the price mentioned above is only a reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs136303
Description	The androgen receptor (AR) is a transcription factor that belongs to the nuclear receptor superfamily and contains zinc finger domains. Activated through phosphorylation and dimerization upon binding to its ligand, AR facilitates nuclear localization and binds to androgen response elements found in target genes involved in androgen signaling. Of particular importance, AR plays a critical role in various aspects of male development and also contributes to the progression of prostate cancer.
Other names	Variant 2 of the androgen nuclear receptor, also known as androgen receptor (AR), plays a crucial role in various conditions such as testicular feminization, Kennedy disease (spinal and bulbar muscular atrophy), and dihydrotestosterone receptor (DHTR). This receptor, encoded by the AR gene, is responsible for binding dihydrotestosterone and regulating androgen hormone signaling. Additionally, the AR splice variant 4b (AR8), also referred to as HYSP1 or SMAX1, has been identified and studied in relation to these conditions. Understanding the function and regulation of the androgen receptor and its various variants is vital for comprehending the mechanisms underlying androgen-related disorders such as TFM and KD. Known as NR3C4 within the nuclear receptor subfamily 3 group C, it plays a significant role in androgen-mediated processes. HUMARA and AR are commonly used names for the androgen receptor, which is a key target for therapeutic interventions aimed at modulating androgen signaling. In conclusion, the androgen receptor and its variants have a profound impact on a range of physiological and pathological processes, making them a subject of extensive research in the field of endocrinology.
Source	Rabbit
Specificity	AR Antibody detects endogenous levels of total AR.
Species Reactivity	Human;Mouse;Rat
Antigen	AR
Application	The dilution ratios for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000. Please note that the generated content should follow a different structure and convey the same information as the original text.
Immunogen	A synthetic peptideof human AR.
MW	110kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Tissue specificity	Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
Posttranslational modification	Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	P10275