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Rabbit Anti-ASCL1 Polyclonal Antibody#abs138024

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Description

Catalog-specification	Delivery time	USD price
abs138024-100ug	1-2 Weeks	301.0
abs138024-50ug	1-2 Weeks	201.0

Overview
catalog	abs138024
Other names	1. The Achaete scute complex homolog 1, also known as Achaete scute complex homolog-like 1 or Achaete scute complex homologue 1, is a transcription factor that belongs to the Achaete scute family of bHLH (basic helix-loop-helix) proteins. It is also referred to as Achaete scute protein, Achaete-scute homolog 1, or ASCL1. 2. Another name for Achaete scute complex homolog 1 is Ash 1 or ASH-1. It is also known as Ash1 or bHLHa46, representing its classification as a Class A basic helix-loop-helix protein, specifically the 46th member in this group. 3. Mammalian achaete scute homolog 1 is a synonymous term used for Achaete scute complex homolog 1. It can also be called Mammalian achaete scute homologue 1 or Mash 1. In summary, the Achaete scute complex homolog 1 is a transcription factor that belongs to the Achaete scute family of bHLH proteins. It is known by various names, including Ash1, ASCL1, and Mash1, and plays a crucial role in gene regulation and development.
Source	Rabbit
Specificity	The total ASCL1 levels are detected by the ASCL1 Antibody, which specifically binds to endogenous ASCL1 protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Dog
Antigen	ASCL1
Application	For Western blotting, a dilution of 1:1000-3000 is recommended. When it comes to immunohistochemistry, a dilution of 1:200 is optimal. For ELISA using a peptide, a dilution of 1:20000-1:40000 is suggested for best results. It is essential to follow the recommended dilutions to obtain accurate and reliable results in all these assays. Dilution factors that are too high or too low can cause variability in the data obtained, leading to erroneous conclusions. Therefore, it is necessary to ensure that the concentrations used are within the specified range for the assays.
Immunogen	A synthesized peptide derived from human ASCL1.
MW	25 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum. To ensure the highest quality of the final product, this method was chosen to remove any impurities and isolate the desired components. The resulting antiserum attained from this purification step is expected to be of the highest purity, and can be used for a wide range of applications.
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the temperature in the storage area is maintained at -20 °C for a period of one year. It is crucial to avoid subjecting the stored items to repeated cycles of freezing and thawing.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation.
Celluar localization	Nucleus;
UniPort	P50553