Phospho-GluR2 (Ser880) Rabbit Polyclonal Antibody#abs130932

abs130932-50ug

1-2 Weeks

201

abs130932-100ug

1-2 Weeks

301

Description

GluR2 is a key membrane protein that belongs to the glutamate-gated ion channel family. Typically, L-glutamate serves as the primary excitatory neurotransmitter at synapses throughout the central nervous system. Within this context, glutamate receptors come in the form of heteromeric protein complexes that consist of multiple subunits, each of which have transmembrane domains and are arranged to form a ligand-gated ion channel. It's also worth noting that the postsynaptic actions of glutamate are mediated by various receptors that are named based on their specific selective agonists.

Other names

AMPA 2, also known as AMPA selective glutamate receptor 2 or AMPA-selective glutamate receptor 2, is a member of the glutamate receptor ionotropic family. It is encoded by the GRIA2 gene in humans and is enriched in the central nervous system, where it plays a crucial role in synaptic plasticity and learning and memory. GluA2 is another name for AMPA 2 and it is also known by the names GLUR 2, GLUR B, GluR K2, GluR-2, GluR-B, and GluR-K2. The GLUR2 gene encodes for the protein that forms the receptor for glutamate, which is the most abundant neurotransmitter in the brain. Because of its critical role in neuronal signaling, mutations in GRIA2 have been linked to neurological disorders such as epilepsy and schizophrenia. Therefore, studying the properties and functions of AMPA 2 is essential for understanding the mechanisms underlying these pathologies and developing effective treatments.

Source

Rabbit

Specificity

The antibody, Phospho-GluR2 (Ser880), specifically identifies the presence of GluR2 protein that has undergone phosphorylation at Serine 880 site. It does not detect non-phosphorylated GluR2 protein. The amount of GluR2 protein present in the sample can be determined by measuring the antibody signal intensity.

Predictive reaction species

Xenopus;Rabbit;Pig;Sheep;Horse;Bovine;Chicken

Application

For Western blot, the recommended dilution range is 1:500 to 1:2000. For immunohistochemistry, the recommended dilution range is 1:50 to 1:200. When performing ELISA with the peptide, it is recommended to dilute 1:20000 to 1:40000. It is important to follow the recommended dilution range for each application to ensure accurate and reproducible results.

A highly similar content can be generated by rearranging the original information as follows: "Serine 880 is an important phosphorylation site found in human GluR2 protein. A synthesized peptide has been derived from GluR2 that specifically targets this phosphorylation site."

Properties

1mg/ml

Purification

The affinity-purified antibody, derived from purified rabbit serum, was obtained through sequential chromatography on affinity columns containing phospho- and non-phospho-peptides. Let's generate a highly similar content by rearranging the above information, while ensuring that it maintains the original text's essence.

Clonality

Polyclonal Antibody

To ensure optimum storage conditions, keep the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is considered stable for a period of 12 months from the receipt date.

Target

Background

 Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Celluar localization

Endoplasmic reticulum;Plasma Membrane;

P42262