Phospho-mTOR (Ser2481) Rabbit Polyclonal Antibody#abs130934

abs130934-50ug

1-2 Weeks

201

abs130934-100ug

1-2 Weeks

301

Description

A unique PIKK kinase is responsible for regulating cell growth by supervising protein synthesis. It functions downstream of the PI3K/Akt pathway and is crucial for the survival of cells. The FKBP12-rapamycin complex targets this kinase to achieve cell-cycle arrest and immunosuppressive effects.

Other names

FK506 binding protein 12 rapamycin associated protein 1, also known as FKBP12-rapamycin complex-associated protein 1 or FRAP1, is a serine/threonine-protein kinase known as mechanistic target of rapamycin or mTOR. It is the primary protein target for the immunosuppressant drug rapamycin and is involved in cellular growth and metabolism regulation.
FKBP12-rapamycin complex-associated protein 2, or FRAP2, is a closely related protein that also binds to rapamycin. Both FKBP12-rapamycin complex-associated proteins play important roles in cell signaling pathways and are key targets for drug development for various diseases.
Other aliases for these proteins include Rapamycin and FKBP12 target 1 (RAFT1), Rapamycin target protein 1 (RAPT1), FKBP rapamycin associated protein, and mammalian target of rapamycin (MTOR). They are also sometimes referred to as OTTHUMP00000001983 and FLJ44809.
Understanding the function and regulation of these proteins is critical for the development of effective therapies for diseases such as cancer, metabolic disorders, and neurological disorders. Their importance in cellular processes has led to extensive research in the field and has resulted in the development of targeted therapies that specifically modulate their activity.

Source

Rabbit

Specificity

The antibody of Phospho-mTOR (Ser2481) is capable of identifying the presence of mTOR in the body only when it is phosphorylated at the specific Serine 2481 site. This detection of endogenous levels of mTOR in the body is based on the level of phosphorylation occurring at this particular site. Hence, the specificity of this antibody lies in its ability to accurately detect mTOR protein levels in the body only when the phosphorylation at Ser2481 is evident.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

WB（1:500-1:2000），IHC（1:50-1:200），IF/ICC（1:100-1:500），（ELISA）（1:20000-1:40000）。，。

mTOR2481，，。

Properties

1mg/ml

Purification

。，。ChapGPT-。

Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity).

Tissue specificity

Expressed in numerous tissues, with highest levels in testis.

Celluar localization

Cytosol;Endoplasmic reticulum;Golgi apparatus;Lysosome;Mitochondrion;Nucleus;

P42345