Phospho-GluR1 (Ser863) Rabbit Polyclonal Antibody#abs130931

abs130931-50ug

1-2 Weeks

201

abs130931-100ug

1-2 Weeks

301

Description

The glutamate-gated ion channel family includes GluR1, a crucial integral membrane protein. Within the central nervous system, L-glutamate (Glu) plays the vital role of an excitatory neurotransmitter at numerous synapses.

Other names

GluR-A, also known as GLUR 1 or AMPA selective glutamate receptor 1, is an important receptor for the neurotransmitter glutamate. It belongs to the ionotropic glutamate receptor family and plays a crucial role in synaptic transmission and plasticity in the brain. GluR-A is encoded by the Gria1 gene and is expressed in various regions of the central nervous system.
The GluR-A receptor is highly selective for the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) subtype of glutamate receptors. It is involved in excitatory neurotransmission and mediates fast synaptic transmission in the brain. GluR-A receptor activation can lead to the influx of calcium ions into the postsynaptic neuron, triggering various signaling cascades and modulating synaptic strength.
Alterations in the expression or function of GluR-A receptors have been implicated in various neurological disorders, including epilepsy, Alzheimer's disease, and schizophrenia. Additionally, GluR-A receptors have been implicated in learning and memory processes, as well as synaptic plasticity, which is vital for neuronal adaptation and development.
In summary, GluR-A, also known as GLUR 1 or AMPA selective glutamate receptor 1, is a crucial receptor involved in synaptic transmission and plasticity. Its specific role in excitatory neurotransmission and its association with various neurological disorders make it an important target for further research and understanding.

Source

Rabbit

Specificity

The Phospho-GluR1 (Ser863) Antibody is specifically designed to recognize the phosphorylated form of GluR1 at Serine 863. This antibody is capable of detecting only the endogenous expression levels of GluR1 protein when it is phosphorylated at this particular site. The specificity of this antibody ensures that any other forms of GluR1 that are not phosphorylated at Serine 863 will not be detected. This antibody is a valuable tool for researchers who are working on investigating the phosphorylation status of GluR1, as well as its role in various cellular processes and diseases. By utilizing this antibody, researchers can gain a deeper understanding of GluR1 function and regulation.

Predictive reaction species

Pig;Rabbit;Sheep;Bovine;Horse

Application

The recommended dilution range for using this antibody in various applications are as follows: for Western blotting (WB), dilute at 1:500 to 1:2000; for immunohistochemistry (IHC), dilute at 1:50 to 1:200; for immunofluorescence/immunocytochemistry (IF/ICC), dilute at 1:100 to 1:500; for ELISA using peptide, dilute the antibody at 1:20000 to 1:40000. Please note that dilution factors may vary based on experimental conditions and the target protein expression levels.

Serine 863 is a crucial phosphorylation site in human GluR1, and scientists have developed a synthesized peptide based on this region. The synthesized peptide closely resembles the original GluR1 sequence around Serine 863, offering immense potential for further research and applications. This innovative approach allows for targeted investigations into the functioning and regulation of GluR1, opening doors to understand its role in various physiological and pathological processes. By utilizing this synthesized peptide, scientists can delve deeper into elucidating the molecular mechanisms underlying GluR1 and its involvement in neurological disorders, ultimately paving the way for the development of novel therapeutic interventions.

Properties

1mg/ml

Purification

The purified rabbit serum was used to isolate the antibody through a process called affinity purification. This involved subjecting the serum to sequential chromatography on both phospho- and non-phospho-peptide affinity columns. By doing so, the targeted antibody was successfully obtained.

Clonality

Polyclonal Antibody

Please make sure to store the item at -20 °C for a duration of one year. It is important to avoid subjecting it to frequent freeze/thaw cycles.

Storage buffer

The storage condition for Rabbit IgG is at -20 °C in a phosphate buffered saline solution with a pH of 7.4. The solution contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product has a shelf life of 12 months from the date of receipt.

Target

Background

 Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Tissue specificity

Widely expressed in brain.

Celluar localization

Endoplasmic reticulum;Endosome;Golgi apparatus;Plasma Membrane;

P42261