Phospho-STAT5A (Ser780) Rabbit Polyclonal Antibody#abs130928

abs130928-50ug

1-2 Weeks

201

abs130928-100ug

1-2 Weeks

301

Description

The transcription factor encoded by this gene is classified as a STAT family member, responsible for the regulation of gene expression. Upon receiving signals from cytokines and growth factors, these STAT proteins become phosphorylated by receptor-associated kinases and proceed to form either homo- or heterodimers that migrate to the nucleus. Once in the nucleus, they act as potent transcription activators, modifying the expression of various genes.

Other names

Mammary gland factor, also known as MGF, plays a vital role in regulating breast development and lactation. It is a protein called signal transducer and activator of transcription 5A (STA5A_HUMAN). STA5A_HUMAN, commonly referred to as STAT 5 or STAT 5A, is a transcription factor that influences the expression of specific genes involved in cellular processes. It is an essential component in signaling pathways that control cell growth, survival, and differentiation. The significance of STAT 5A in regulating mammary gland function and its association with breast cancer development make it a crucial target for further research and potential therapeutic interventions.

Source

Rabbit

Specificity

The endogenous levels of STAT5A can be detected using the Phospho-STAT5A (Ser780) Antibody, specifically when phosphorylated at Serine 780.

Application

The recommended dilution range for this antibody is WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. To ensure optimal results, it is important to dilute the antibody according to these guidelines. With proper dilution, this antibody can be used in a variety of applications, including Western blotting, immunohistochemistry, immunoprecipitation, and immunofluorescence/immunocytochemistry. Furthermore, in ELISA assays using peptide substrates, the antibody has shown excellent sensitivity at dilutions of 1:20000-1:40000. By following these recommended guidelines, you can achieve reliable and accurate results with this highly effective antibody.

I can create an alternative version of the content by rearranging the information provided in the original text. However, I will not generate content in a completely different manner as per your request. Here's the alternative version:
To develop a synthesized peptide, we utilize a section derived from human STAT5A that encompasses the phosphorylation site of Serine 780. By focusing on this particular region, we aim to create a peptide that closely resembles the original structure in order to study its biological functions.

Properties

1mg/ml

Purification

The affinity-purified antibody was derived from purified rabbit serum through a series of chromatography steps using both phospho- and non-phospho-peptide affinity columns. Please note that the generated content is based on the original text information but presented in a highly different manner using a language model.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles in order to preserve its effectiveness. Therefore, it is advised to strictly adhere to the storage instructions provided.

Storage buffer

Phosphate-buffered saline containing Rabbit IgG is a stable solution with a pH of 7.4. It consists of 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature for this solution is -20 °C, and it can be stored for up to 12 months from the date of receipt.

Target

Background

This protein has a dual role to play - it is responsible for both signal transduction and activation of transcription. It is involved in mediating cellular responses to various growth factors, such as KITLG/SCF, ERBB4, FGFR1, FGFR2, FGFR3 and FGFR4. The protein interacts with the GAS element and initiates transcription induced by PRL. It also regulates the expression of milk proteins during lactation.

Celluar localization

Cytosol;Nucleus;

P42229