Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-PAM Polyclonal Antibody#abs137705

Please note that the price mentioned is only for your reference. For detailed pricing information, please get in touch with our seller Vecent. It is important to keep in mind that the final cost may vary based on a range of factors. Therefore, we recommend that you reach out to Vecent directly...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs137705-100ug	1-2 Weeks	301.0
abs137705-50ug	1-2 Weeks	201.0

Overview
catalog	abs137705
Other names	Pancreatic peptidylglycine alpha amidating monooxygenase, abbreviated as PAM, is an enzyme responsible for the amidation of peptide hormones and neuropeptides. It plays a crucial role in the post-translational modification of these molecules by catalyzing the conversion of peptidyl-alpha-hydroxyglycine to alpha-amidated peptides. As a peptidyl alpha amidating enzyme, PAM functions through a series of biochemical reactions. It hydroxylates the alpha-carbon of peptidylglycine, creating an unstable intermediate known as peptidyl-alpha-hydroxyglycine. Then, it further catalyzes the conversion of this intermediate into alpha-amidated peptides by cleaving off the peptide portion and replacing it with an amide group. This process is commonly referred to as amidation and is vital for the bioactivity, stability, and targeting of peptide hormones. Another name for PAM is peptidyl alpha hydroxyglycine alpha-amidating lyase. This name reflects its dual function as both a hydroxylase and a lyase enzyme. It hydroxylates the alpha-carbon of peptidylglycine and subsequently cleaves the peptide bond to form an amide group. In some literature, PAM is also referred to as peptidylamidoglycolate lyase. This name emphasizes its role in converting peptidyl-alpha-hydroxyglycine into amidated peptides by lyase activity. Peptidylglycine 2 hydroxylase, on the other hand, is a closely related enzyme that shares similarities with PAM. It also catalyzes the hydroxylation of peptidylglycine but lacks the amidating lyase activity. This enzyme is involved in the production of alpha-hydroxylated peptides, which have distinct biological functions from amidated peptides. Overall, PAM is an essential enzyme involved in the amidation process of peptide hormones and neuropeptides. Its intricate catalytic reactions are crucial for the production of biologically active peptides necessary for various physiological processes in the human body.
Source	Rabbit
Specificity	The total PAM levels can be efficiently detected by the PAM Antibody, which specifically targets the endogenous PAM protein.
Species Reactivity	Human;Mouse;Rat
Antigen	PAM
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human PAM.
MW	108 kDa
Properties
Concentration	1mg/ml
purification	We employed SulfoLink™ Coupling Resin for peptide affinity chromatography in order to purify the antiserum. Can you provide more specific information or context about the antiserum purification process I can assist with?
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the preservation of the product, it is recommended to store it at -20 °C for a period of one year. To avoid any potential damage, it is important to refrain from subjecting the product to repeated freeze/thaw cycles.
Storage buffer	IgG from rabbits is dissolved in phosphate buffered saline with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to store this solution at -20 °C. The solution remains stable for a period of 12 months from the date it is received.
Target
Background	Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
Celluar localization	Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P19021